Evidence map›Paper›PMID 35551498›Full record

ArticleCommunications biology2022

3D molecular phenotyping of cleared human brain tissues with light-sheet fluorescence microscopy.

Luca Pesce, Marina Scardigli, Vladislav Gavryusev, Annunziatina Laurino, Giacomo Mazzamuto, Niamh Brady, Giuseppe Sancataldo, Ludovico Silvestri, Christophe Destrieux, Patrick R Hof and 2 more

Open access · goldAbstract read
In one paragraph

Article in Communications biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
12.4field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 50 citations in OpenAlex.

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  14. T-CLEARE: a pilot community-driven tissue clearing protocol repository.Frontiers in bioengineering and biotechnology · 2024
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 3 countries.

Luca PesceEuropean Laboratory for Non-linear Spectroscopy (LENS), University of Florence, Sesto Fiorentino, Italy.ORCID http://orcid.org/0000-0002-5863-399X
Marina ScardigliEuropean Laboratory for Non-linear Spectroscopy (LENS), University of Florence, Sesto Fiorentino, Italy.
Vladislav GavryusevEuropean Laboratory for Non-linear Spectroscopy (LENS), University of Florence, Sesto Fiorentino, Italy.ORCID http://orcid.org/0000-0001-7734-7828
Annunziatina LaurinoEuropean Laboratory for Non-linear Spectroscopy (LENS), University of Florence, Sesto Fiorentino, Italy.
Giacomo MazzamutoEuropean Laboratory for Non-linear Spectroscopy (LENS), University of Florence, Sesto Fiorentino, Italy.ORCID http://orcid.org/0000-0003-3077-3904
Niamh BradyEuropean Laboratory for Non-linear Spectroscopy (LENS), University of Florence, Sesto Fiorentino, Italy.
Giuseppe SancataldoEuropean Laboratory for Non-linear Spectroscopy (LENS), University of Florence, Sesto Fiorentino, Italy.
Ludovico SilvestriEuropean Laboratory for Non-linear Spectroscopy (LENS), University of Florence, Sesto Fiorentino, Italy.ORCID http://orcid.org/0000-0001-9215-9104
Christophe DestrieuxUMR 1253, iBrain, Université de Tours, Tours, France.
Patrick R HofNash Family Department of Neuroscience and Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Irene CostantiniEuropean Laboratory for Non-linear Spectroscopy (LENS), University of Florence, Sesto Fiorentino, Italy. costantini@lens.unifi.it.ORCID http://orcid.org/0000-0002-8464-7324
Francesco S PavoneEuropean Laboratory for Non-linear Spectroscopy (LENS), University of Florence, Sesto Fiorentino, Italy.
University of Florence · ITNational Research Council · ITAllen Institute for Brain Science · USUniversité de Tours · FR

Funding

Imaging and Analysis Techniques to Construct a Cell Census Atlas of the Human Brain Admin SupplementU01MH117023 · NIMH · MASSACHUSETTS GENERAL HOSPITAL · PI BOAS, DAVID A, FISCHL, BRUCE · 2018 to 2022
$7.6M
NIMH NIH HHS U01 MH117023
6 · The paper itself

Abstract

The combination of optical tissue transparency with immunofluorescence allows the molecular characterization of biological tissues in 3D. However, adult human organs are particularly challenging to become transparent because of the autofluorescence contributions of aged tissues. To meet this challenge, we optimized SHORT (SWITCH-H

Indexed as

Hydrogen PeroxideImaging, Three-DimensionalAdultAgedBrainFluorescent Antibody TechniqueHumansMicroscopy, FluorescenceHydrogen Peroxide

Identifiers

PMID35551498
PMCPMC9098858
OpenAlexW4280585084

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.