Evidence map›Paper›PMID 35551198›Full record

ArticleScientific reports2022

Preyssler-type phosphotungstate is a new family of negative-staining reagents for the TEM observation of viruses.

Koichi Sahiro, Yasuhiko Kawato, Kanae Koike, Tsuneji Sano, Toshihiro Nakai, Masahiro Sadakane

Abstract read
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Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
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  5. NewPHAGE (New Rochelle, N.Y.) · 2025
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Koichi SahiroDepartment of Applied Chemistry, Graduate School of Advanced Science and Engineering, Hiroshima University, 1-4-1 Kagamiyama, Higashi-Hiroshima, 739-8527, Japan.
Yasuhiko KawatoPathology Division, Nansei Field Station, Fisheries Technology Institute, Japan Fisheries Research and Education Agency, 516-0193, Minami-Ise, Japan.
Kanae KoikeNatural Science Center for Basic Research and Development, Hiroshima University, 1-4-2 Kagamiyama, Higashi-Hiroshima, 739-8527, Japan.
Tsuneji SanoDepartment of Applied Chemistry, Graduate School of Advanced Science and Engineering, Hiroshima University, 1-4-1 Kagamiyama, Higashi-Hiroshima, 739-8527, Japan.
Toshihiro NakaiTakehara Marine Science Station, Graduate School of Integrated Science for Life, Hiroshima University, Takehara, 725-0024, Japan.
Masahiro SadakaneDepartment of Applied Chemistry, Graduate School of Advanced Science and Engineering, Hiroshima University, 1-4-1 Kagamiyama, Higashi-Hiroshima, 739-8527, Japan. sadakane09@hiroshima-u.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Transmission electron microscopy (TEM) is an essential method in virology because it allows for direct visualization of virus morphology at a nanometer scale. Negative staining to coat virions with heavy metal ions must be performed before TEM observations to achieve sufficient contrast. Herein, we report that potassium salts of Preyssler-type phosphotungstates (K

Indexed as

SaltsVirusesIndicators and ReagentsIonsMicroscopy, Electron, TransmissionNegative StainingPotassiumStaining and LabelingIndicators and ReagentsIonsPotassiumSalts

Identifiers

PMID35551198
PMCPMC9098496

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.