ArticleFrontiers in plant science2022
Expanding the Editing Window of Cytidine Base Editors With the Rad51 DNA-Binding Domain in Rice.
Article in Frontiers in plant science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed.
- Synergistic optimization enhancing the precision and efficiency of cytosine base editors in poplar.Communications biology · 2025Article
- Genetic engineering of RuBisCO by multiplex CRISPR editing small subunits in rice.Plant biotechnology journal · 2025Article
- In silico structural analysis of Oryza sativa RAD51 reveals key interactions for nucleoprotein filament assembly and regulation.PloS one · 2025Article
- An SpG-Cas9-based cytosine base editor expands the scope of genome editing in carrot plants.Plant cell reports · 2024Article
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9 authors.
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Abstract
Recently developed base editors provide a powerful tool for plant research and crop improvement. Although a number of different deaminases and Cas proteins have been used to improve base editors the editing efficiency, and editing window are still not optimal. Fusion of a non-sequence-specific single-stranded DNA-binding domain (DBD) from the human Rad51 protein between Cas9 nickase and the deaminase has been reported to dramatically increase the editing efficiency and expand the editing window of base editors in the mammalian cell lines and mouse embryos. We report the use of this strategy in rice, by fusing a rice codon-optimized human Rad51 DBD to the cytidine base editors AncBE4max, AncBE4max-NG, and evoFERNY. Our results show that the addition of Rad51 DBD did not increase editing efficiency in the major editing window but the editing range was expanded in all the three systems. Replacing the human Rad51 DBD with the rice Rad51 DBD homolog also expanded the editing window effectively.
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