ArticleRSC advances2018
Green and efficient biosynthesis of indigo from indole by engineered myoglobins.
Article in RSC advances, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- Less is more: miniaturized heme enzymes with broad reactivity.Chemical science · 2026Review
- Biocatalytic Indigo Synthesis From L-Tryptophan Using a Three-Step Cascade Without Cofactor Regeneration.Chembiochem : a European journal of chemical biology · 2026Article
- Mechanochemistry Reaches-Out Sensing: Peroxidase-Mimic Fe-BTC MOF for Hydrogen Peroxide Detection by a Solvent-Free Synthesis.ACS omega · 2025Article
- Biohybrid materials comprising an artificial peroxidase and differently shaped gold nanoparticles.Nanoscale advances · 2024Article
- Biosynthesis of High-Active Hemoproteins by the Efficient Heme-Supply Pichia Pastoris Chassis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Dye Decolorization by a Miniaturized Peroxidase Fe-MimochromeVI*a.International journal of molecular sciences · 2023Article
- Construction of Biocatalysts Using the P450 Scaffold for the Synthesis of Indigo from Indole.International journal of molecular sciences · 2023Article
- Discovery of New Phenylacetone Monooxygenase Variants for the Development of Substituted Indigoids through Biocatalysis.International journal of molecular sciences · 2022Article
- Designing Artificial Metalloenzymes by Tuning of the Environment beyond the Primary Coordination Sphere.Chemical reviews · 2022Review
- A nano-sized Cu-MOF with high peroxidase-like activity and its potential application in colorimetric detection of HRSC advances · 2021Article
- Production of Tyrian purple indigoid dye from tryptophan in Escherichia coli.Nature chemical biology · 2021Article
- Biotransformation of Lignin by an Artificial Heme Enzyme Designed in Myoglobin With a Covalently Linked Heme Group.Frontiers in bioengineering and biotechnology · 2021Article
- Photo induced reaction of myoglobins with energy transferred from excited free tryptophan.RSC advances · 2020Article
- Rational Design of Artificial Metalloproteins and Metalloenzymes with Metal Clusters.Molecules (Basel, Switzerland) · 2019Review
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
With the demand nowadays for blue dyes, it is of practical importance to develop a green and efficient biocatalyst for the production of indigo. The design of artificial enzymes has been shown to be attractive in recent years. In a previous study, we engineered a single mutant of sperm whale myoglobin, F43Y Mb, with a novel Tyr-heme cross-link. In this study, we found that it can efficiently catalyze the oxidation of indole to indigo, with a yield as high as 54% compared to the highest yield (∼20%) reported to date in the literature. By further modifying the heme active site, we engineered a double mutant of F43Y/H64D Mb, which exhibited the highest catalytic efficiency (198 M
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