Evidence map›Paper›PMID 35546794›Full record

ArticleRSC advances2018

Highly efficient soluble expression, purification and characterization of recombinant Aβ42 from

Longgang Jia, Wenjuan Wang, Jinzhao Shang, Wenping Zhao, Wei Wei, Ying Wang, Li Li, Fuping Lu, Fufeng Liu

Open access · goldAbstract read
In one paragraph

Article in RSC advances, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.8field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 11 citations in OpenAlex.

  1. Review
  2. Article
  3. Characterization and insight mechanism of an acid-adapted β-Glucosidase fromFrontiers in bioengineering and biotechnology · 2024
    Article
  4. Unveiling the therapeutic potential ofFrontiers in pharmacology · 2024
    Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 2 countries.

Longgang JiaState Key Laboratory of Food Nutrition and Safety (Tianjin University of Science & Technology) Tianjin 300457 P. R. China fufengliu@tust.edu.cn +86-22-60602298 +86-22-60602717.ORCID https://orcid.org/0000-0003-3400-5531
Wenjuan WangState Key Laboratory of Food Nutrition and Safety (Tianjin University of Science & Technology) Tianjin 300457 P. R. China fufengliu@tust.edu.cn +86-22-60602298 +86-22-60602717.
Jinzhao ShangState Key Laboratory of Food Nutrition and Safety (Tianjin University of Science & Technology) Tianjin 300457 P. R. China fufengliu@tust.edu.cn +86-22-60602298 +86-22-60602717.
Wenping ZhaoState Key Laboratory of Food Nutrition and Safety (Tianjin University of Science & Technology) Tianjin 300457 P. R. China fufengliu@tust.edu.cn +86-22-60602298 +86-22-60602717.
Wei WeiState Key Laboratory of Food Nutrition and Safety (Tianjin University of Science & Technology) Tianjin 300457 P. R. China fufengliu@tust.edu.cn +86-22-60602298 +86-22-60602717.
Ying WangState Key Laboratory of Food Nutrition and Safety (Tianjin University of Science & Technology) Tianjin 300457 P. R. China fufengliu@tust.edu.cn +86-22-60602298 +86-22-60602717.
Li LiCollege of Marine and Environmental Sciences, Tianjin University of Science & Technology Tianjin 300457 P. R. China.
Fuping LuState Key Laboratory of Food Nutrition and Safety (Tianjin University of Science & Technology) Tianjin 300457 P. R. China fufengliu@tust.edu.cn +86-22-60602298 +86-22-60602717.
Fufeng LiuState Key Laboratory of Food Nutrition and Safety (Tianjin University of Science & Technology) Tianjin 300457 P. R. China fufengliu@tust.edu.cn +86-22-60602298 +86-22-60602717.ORCID https://orcid.org/0000-0002-9454-2382
Tianjin University of Science and Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Aggregation of amyloid-β protein (Aβ) is hypothesized to be a seminal neuropathological event in Alzheimer's disease (AD). Recombinant expression and purification of Aβ represents a common basis for investigating the molecular mechanisms of amyloid formation and toxicity. Herein, we report a novel high-yield expression and purification method for Aβ42 based on fusion with maltose binding protein (MBP) followed by the soluble polypeptide linker (NANP)

Identifiers

PMID35546794
PMCPMC9087987
OpenAlexW2804283711

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.