ArticleParasites & vectors2022
Giardia duodenalis in patients with diarrhea and various animals in northeastern China: prevalence and multilocus genetic characterization.
Article in Parasites & vectors, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 2 of them syntheses that pooled it.
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Who cites it
20 citing papers in PubMed, 2 syntheses or guidelines pooled it, 28 citations in OpenAlex.
- Giardia duodenalis in Rodents: A Global Systematic Review and Meta-Analysis.Veterinary medicine and science · 2025Pooled it
- Global prevalence of Giardia infection in nonhuman mammalian hosts: A systematic review and meta-analysis of five million animals.PLoS neglected tropical diseases · 2025Pooled it
- First joint report on the prevalence and genotyping of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi among hospitalized children in Inner Mongolia, China.BMC infectious diseases · 2026Article
- Beyond the clinical eye: mapping intestinal parasitic infections and its risk factors among dogs and cats across Portugal.Frontiers in veterinary science · 2026Article
- First molecular identification and genetic diversity of Giardia duodenalis and Blastocystis sp. in patients with multiple sclerosis in Iran.Molecular biology reports · 2025Article
- Assessing the public health and zoonotic impacts ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2025Article
- Prevalence and assemblage distribution ofFrontiers in veterinary science · 2025Article
- Molecular characterization and risk analysis of Giardia duodenalis assemblages in corticosteroid-treated and non-treated patients in Ismailia, Arab Republic of Egypt.Gut pathogens · 2024Article
- Molecular Detection and Characterization of Cryptosporidium spp. and Giardia duodenalis in Dairy Calves from Ningxia, China.Acta parasitologica · 2024Article
- Wastewater-based intestinal protozoa monitoring in Shanghai, China.Microbiology spectrum · 2024Article
- Infectious etiology and indicators of malabsorption or intestinal injury in childhood diarrhea.Biomedica : revista del Instituto Nacional de Salud · 2024Article
- Prevalence and assemblage identified of Giardia duodenalis in zoo and farmed Asiatic black bears (Ursus thibetanus) from the Heilongjiang and Fujian Provinces of China.Parasite (Paris, France) · 2024Article
- Molecular Epidemiology and Assemblage Typing of Giardia duodenalis in School-Age Children Situated along the Southern Shoreline of Lake Malawi, Malawi.The American journal of tropical medicine and hygiene · 2023Article
- Influence of Selected Factors on the Survival Assessment and Detection ofPathogens (Basel, Switzerland) · 2023Article
- Molecular identification and genetic characteristics of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in human immunodeficiency virus/acquired immunodeficiency syndrome patients in Shanghai, China.Parasites & vectors · 2023Article
- Review
- The detection and phylogenetic characterization ofFrontiers in cellular and infection microbiology · 2023Article
- Giardia lamblia-infected preschoolers present growth delays independent of the assemblage A, B or E.Memorias do Instituto Oswaldo Cruz · 2023Article
- Molecular characterisation ofCurrent research in parasitology & vector-borne diseases · 2022Article
- Molecular detection and genotyping ofTropical parasitologyArticle
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundGiardia duodenalis is a common parasitic diarrheal agent in humans, especially in developing countries. The aim of this study was to investigate the prevalence and multilocus genetic characterization of G. duodenalis in patients with diarrhea and animals in northeastern China, and to assess the epidemiological role of animals in the transmission of human giardiasis.
methodsA total of 1739 fecal specimens from 413 diarrheal patients and 1326 animals comprising 16 mammal species were collected in Heilongjiang Province of China and screened for G. duodenalis by PCR and sequencing of the SSU rRNA gene. All G. duodenalis-positive specimens were subtyped by PCR and sequencing of the bg, tpi, and gdh genes. To detect additional mixed infections of different assemblages, assemblage A/B/E-specific PCRs were performed to amplify the tpi gene.
resultsSequence analysis of the SSU rRNA gene determined the prevalence of G. duodenalis (5.81%, 24/413) in diarrheal patients, with a peak in minors aged 5-17 years, and identified assemblages A and B. MLG-AII and MLG-B1 were obtained based on concatenated nucleotide sequences of the bg, tpi, and gdh genes, with MLG-AII being identical to a cat-derived isolate reported previously. By sequence analysis of the SSU rRNA gene, G. duodenalis was detected in 214 (16.14%) animals belonging to 11 mammal species, with the prevalence ranging from 1.69 to 53.85%, and assemblages A to G were identified. Sequence analysis of the bg, tpi, and gdh genes from 46 specimens produced 31 MLGs, including MLG-AI (n = 1), MLG-B2-B8 (n = 18), and MLG-E1-E23 (n = 27).
conclusionsThe finding of G. duodenalis in diarrheal patients enhances consciousness of detecting G. duodenalis in clinical practice and emphasizes the importance of health education in local inhabitants, especially in the age group of 5-17 years. The identification of seven assemblages (A to G) and 33 MLGs reveals genetic heterogeneity of G. duodenalis in the investigated areas. Due to insufficient homology data on the zoonotic transmission of G. duodenalis, the precise epidemiological role that animals play in the transmission of human giardiasis needs to be assessed by more large-scale molecular epidemiological investigations of local humans and animals.
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