ArticleNature communications2022
Metabolite trafficking enables membrane-impermeable-terpene secretion by yeast.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 25 citations in OpenAlex.
- Multi-level metabolic engineering for squalene production: from pathway optimization to systematic regulation.World journal of microbiology & biotechnology · 2026Review
- Research progress on metabolic engineering for efficient squalene synthesis in yeast cell factories.Biotechnology letters · 2026Review
- Dual-channel optogenetics in yeast for multiplexed light-based control of cellular processes and pathways.Nature communications · 2026Article
- EngineeredJournal of fungi (Basel, Switzerland) · 2026Article
- Advances in mitochondrial compartmentalization for terpenoid synthesis inFrontiers in bioengineering and biotechnology · 2026Review
- Acetyl-CoA carboxylase maintains energetic balance for functional oogenesis.Nature communications · 2025Article
- Metabolic Engineering of Yeasts for the Production of the Triterpene Squalene: Current Status and Future Prospective.Microorganisms · 2025Review
- Multidimensional Optimization ofBiodesign research · 2024Article
- EngineeringSynthetic and systems biotechnology · 2023Article
- Article
- Dynamic environmental interactions shaped by vegetative plant volatiles.Natural product reports · 2023Review
- Human lipocalins bind and export fatty acids through the secretory pathway of yeast cells.Frontiers in microbiology · 2023Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metabolites are often unable to permeate cell membranes and are thus accumulated inside cells. We investigate whether engineered microbes can exclusively secrete intracellular metabolites because sustainable metabolite secretion holds a great potential for mass-production of high-value chemicals in an efficient and continuous manner. In this study, we demonstrate a synthetic pathway for a metabolite trafficking system that enables lipophilic terpene secretion by yeast cells. When metabolite-binding proteins are tagged with signal peptides, metabolite trafficking is highly achievable; loaded metabolites can be precisely delivered to a desired location within or outside the cell. As a proof of concept, we systematically couple a terpene-binding protein with an export signal peptide and subsequently demonstrate efficient, yet selective terpene secretion by yeast (~225 mg/L for squalene and ~1.6 mg/L for β-carotene). Other carrier proteins can also be readily fused with desired signal peptides, thereby tailoring different metabolite trafficking pathways in different microbes. To the best of our knowledge, this is the most efficient cognate pathway for metabolite secretion by microorganisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.