ArticleThe Journal of international medical research2022
Effects of sperm preparation techniques on sperm survivability and DNA fragmentation.
Article in The Journal of international medical research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 12 citations in OpenAlex.
- Sequencing Matters: Comparative Effectiveness of Density-Gradient Centrifugation and MACS for Enhancing Sperm Quality and DNA Integrity.Journal of clinical medicine · 2026Article
- Microfluidic Sorting Can Be Applied for Assisted Reproduction Sperm Selection in Different Cases of Semen Abnormalities.Life (Basel, Switzerland) · 2025Article
- Sperm Migration and Hyaluronic Acid Binding: Implications for Male Fertility Evaluation.International journal of molecular sciences · 2024Article
- Sperm quality metrics were improved by a biomimetic microfluidic selection platform compared to swim-up methods.Microsystems & nanoengineering · 2023Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveThis study was performed to determine the effect of swim-up (SU) and density gradient centrifugation (DGC) on sperm survival and DNA fragmentation.
methodsIndividual semen samples were analyzed before each was divided into two aliquots (half for SU and half for DGC) for calculation of sperm survival and the DNA fragmentation index (DFI). Sperm DNA fragmentation was determined using the sperm chromatin dispersion test.
resultsThe DFI of the 63 semen samples processed using both procedures was lower than that of the fresh semen samples. The DFI was significantly lower for samples processed using the SU than DGC method. In the sperm survival test, the SU technique was associated with increased sperm motility and vitality following preparation. After 24 hours, however, the concentration and percentage of surviving sperm were significantly lower in the SU than DGC group.
conclusionsBoth semen preparation techniques help to minimize sperm DNA fragmentation; however, when the DFI is <30%, the SU technique is more appropriate than DGC. While DGC may be superior for intrauterine insemination, the SU method may be preferable for in vitro fertilization or maturation.
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Registered trials
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