ArticleCell reports2022
Reduced housing density improves statistical power of murine gut microbiota studies.
Article in Cell reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 37 citations in OpenAlex.
- Pooled it
- Towards optimizing the host response to biotics: a report of an International Scientific Association for Probiotics and Prebiotics working group.Gut microbes · 2026Article
- A systematic assessment of the short- and long-term effects of commonly used breast cancer chemotherapeutics on the gut microbiome‒blood‒brain axis of female mice.Gut microbes · 2026Article
- Both donor and recipient sex determine behavioral and neuroimmune outcomes in a gut microbiota transfer experiment from the unpredictable chronic mild stress model of depression.Brain, behavior, & immunity - health · 2026Article
- Article
- A Novel Focal Duodenal Radiation Injury Model Reveals Dose-, Time-, and Spatially Dependent Microbiome Perturbations After Radiation Injury.International journal of radiation oncology, biology, physics · 2026Article
- The Effect of Dietary Fiber Compositions on the Therapeutic Outcome of Combined Radio- and Immunotherapy in a Preclinical Cancer Model.Molecular nutrition & food research · 2026Article
- Biological and technical variability in mouse microbiota analysis and implications for sample size determination.Lab animal · 2026Article
- Animal models of gut microbiota-male reproductive interactions: methodological assessment, translational bottlenecks, and optimization strategies.Frontiers in endocrinology · 2026Review
- Housing matters: Experimental variables shaping metabolism in obese mice.Molecular metabolism · 2025Article
- Inducible, but not constitutive, pancreatic REG/Reg isoforms are regulated by intestinal microbiota and pancreatic diseases.Mucosal immunology · 2025Article
- Progressive gut microbiota shifts and functional alterations across aging stages and frailty in mice.iScience · 2025Article
- Modulating effects of environmental enrichment on stress-induced changes in the gut microbiome.Brain, behavior, & immunity - health · 2025Article
- Intestinal microbiota profiles of captive-bred cynomolgus macaques reveal influence of biogeography and age.Animal microbiome · 2025Article
- Dextran sodium sulfate-induced colitis alters the proportion and composition of replicating gut bacteria.mSphere · 2025Article
- Gut bacterium Intestinimonas butyriciproducens improves host metabolic health: evidence from cohort and animal intervention studies.Microbiome · 2025Article
- Expanding the use of circulating microbiome in fish: contrast between the gut and blood microbiome ofISME communications · 2025Article
- Integrated Metagenomic and Metabolomic Analysis of In Vitro Murine Gut Microbial Cultures upon Bisphenol S Exposure.Metabolites · 2024Article
- Symposium: What Does the Microbiome Tell Us about Prevention and Treatment of AD/ADRD?The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Review
- A Clinical Scoring System and Impact of Athymic Nude Mouse Age onComparative medicine · 2024Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
The gut microbiome of humans and animals is critical to host health. Mice are used to investigate the microbiome and its influences; however, the predictive value of such studies is hindered by cage effects due to coprophagy. Our objectives were to evaluate the influence of cage density on the statistical power to detect treatment-dependent effects of a selective pressure on microbiome composition. C57BL/6 mice were separated into groups of 2 or 4 mice per cage and then assigned to groups receiving enrofloxacin, broad-spectrum antibiotics, or control. Fecal samples were collected at weeks 0, 1, and 4, along with contents of the jejunum and cecum. Bacterial DNA analysis examined microbiome richness, diversity, and variability within and between cages. Statistical analyses reveal that reduced housing density consistently results in comparable susceptibility to antibiotics, reduced cage effects, and increased statistical power to detect treatment-associated effects, justifying the practice of reduced housing density.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.