Evidence map›Paper›PMID 35543537›Full record

ArticleJournal of bacteriology2022

Cell Wall Damage Reveals Spatial Flexibility in Peptidoglycan Synthesis and a Nonredundant Role for RodA in Mycobacteria.

Emily S Melzer, Takehiro Kado, Alam García-Heredia, Kuldeepkumar Ramnaresh Gupta, Xavier Meniche, Yasu S Morita, Christopher M Sassetti, E Hesper Rego, M Sloan Siegrist

Abstract read
In one paragraph

Article in Journal of bacteriology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed.

  1. Defining the order of assembly of theJournal of bacteriology · 2026
    Article
  2. Article
  3. Defining the order of assembly of thebioRxiv : the preprint server for biology · 2026
    Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. TheFrontiers in microbiology · 2025
    Article
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  12. Review
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  18. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Emily S MelzerDepartment of Microbiology, University of Massachusetts, Amherst, Massachusetts, USA.ORCID 0000-0001-5489-0975
Takehiro KadoDepartment of Microbiology, University of Massachusetts, Amherst, Massachusetts, USA.
Alam García-HerediaMolecular and Cellular Biology Graduate Program, University of Massachusetts, Amherst, Massachusetts, USA.
Kuldeepkumar Ramnaresh GuptaDepartment of Microbial Pathogenesis, Yale University School of Medicine, New Haven, Connecticut, USA.
Xavier MenicheDepartment of Microbiology and Physiological Systems, University of Massachusetts Medical Schoolgrid.168645.8, Worcester, Massachusetts, USA.
Yasu S MoritaDepartment of Microbiology, University of Massachusetts, Amherst, Massachusetts, USA.ORCID 0000-0002-4514-9242
Christopher M SassettiDepartment of Microbiology and Physiological Systems, University of Massachusetts Medical Schoolgrid.168645.8, Worcester, Massachusetts, USA.
E Hesper RegoDepartment of Microbial Pathogenesis, Yale University School of Medicine, New Haven, Connecticut, USA.ORCID 0000-0002-2973-8354
M Sloan SiegristDepartment of Microbiology, University of Massachusetts, Amherst, Massachusetts, USA.ORCID 0000-0002-8232-3246

Funding

CHEMISTRY-BIOLOGY INTERFACE PREDOCTORAL TRAINING GRANTST32GM008515 · NIGMS · UNIVERSITY OF MASSACHUSETTS AMHERST · PI THOMPSON, LYNMARIE K. · 1995 to 2020
$4.3M
Chemistry-Biology Interface Predoctoral Training GrantT32GM139789 · NIGMS · UNIVERSITY OF MASSACHUSETTS AMHERST · PI ERIC Robert STRIETER · 2021 to 2026
$3.4M
Chemical detection of Mycobacterium tuberculosis growth and antibiotic response during infectionDP2AI138238 · NIAID · UNIVERSITY OF MASSACHUSETTS AMHERST · PI SIEGRIST, MARY SLOAN · 2017 to 2017
$2.3M
"The molecular mechanisms of asymmetric cell division in mycobacteria."R01AI148255 · NIAID · YALE UNIVERSITY · PI REGO, ELIZABETH HESPER · 2020 to 2024
$2.1M
Spatial organization of mycobacterial cell wall biosynthesisR21AI144748 · NIAID · UNIVERSITY OF MASSACHUSETTS AMHERST · PI MORITA, YASU S, SIEGRIST, MARY SLOAN · 2019 to 2020
$423k
NIAID NIH HHS DP2 AI138238NIAID NIH HHS R01 AI148255NIAID NIH HHS R21 AI144748NIGMS NIH HHS T32 GM008515NIGMS NIH HHS T32 GM139789
6 · The paper itself

Abstract

Cell wall peptidoglycan is a heteropolymeric mesh that protects the bacterium from internal turgor and external insults. In many rod-shaped bacteria, peptidoglycan synthesis for normal growth is achieved by two distinct pathways: the Rod complex, comprised of MreB, RodA, and a cognate class B penicillin-binding protein (PBP), and the class A PBPs (aPBPs). In contrast to laterally growing bacteria, pole-growing mycobacteria do not encode an MreB homolog and do not require SEDS protein RodA for

Indexed as

Bacterial ProteinsPeptidoglycanAnimalsCell WallMycobacterium smegmatisPenicillin-Binding ProteinsBacterial ProteinsPenicillin-Binding ProteinsPeptidoglycancell envelopecell wallmycobacteriapeptidoglycanstress responsetuberculosis

Identifiers

PMID35543537
PMCPMC9210966

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.