ArticleRSC advances2019
A novel method of sample homogenization with the use of a microtome-cryostat apparatus.
Article in RSC advances, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Nanosecond Infrared Laser Sampling of Mouse and Human Liver Tissues for LC-MS/MS Analysis of Bile Acids.International journal of molecular sciences · 2026Article
- Integrating metabolomics and histopathology: A method for metabolite recovery from fixed tissue specimens.Structural dynamics (Melville, N.Y.) · 2026Article
- Systematic evaluation of pre-analytical variables on synovial fluid metabolomic profiles using GC-ToF-MS and UHPLC-MS.The Analyst · 2026Article
- Impact of soft tissue homogenization methods on RNA quality.Molecular biology reports · 2025Article
- Methodological Developments for Metabolic NMR Spectroscopy from Cultured Cells to Tissue Extracts: Achievements, Progress and Pitfalls.Molecules (Basel, Switzerland) · 2022Review
- Post-mortem changes in metabolomic profiles of human serum, aqueous humor and vitreous humor.Metabolomics : Official journal of the Metabolomic Society · 2020Article
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Authors and funding
3 authors.
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Abstract
Quantitative metabolomics places high demands on sample preparation, including a high degree of metabolite extraction and controlled sample weight. In respect to elastic collagen-rich tissues, the existing methods of sample homogenization poorly fit these demands due to incomplete homogenization, sample material loss, or metabolite degradation. Herein, a novel method based on the use of a microtome-cryostat apparatus is proposed. The performance of the cryotome method is compared with the results obtained with the use of a vortex bead beating. NMR-based metabolomic analysis shows that the extraction efficiency and the data scattering for both methods of sample preparation are similar. However, the heat generation during the bead beating causes the destruction of thermally-unstable compounds; besides, it may cause protein hydrolysis, leading to an artificial increase in the amino acid level. The cryotome method of sample homogenization does not cause sample heating, and it seems to be ideal for elastic tissues.
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Registered trials
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