Evidence map›Paper›PMID 35541422›Full record

ArticleRSC advances2019

Multiplex detection of miRNAs based on aggregation-induced emission luminogen encoded microspheres.

Dan Zou, Weijie Wu, Jingpu Zhang, Qiang Ma, Sisi Fan, Jin Cheng, Dan Li, Jiaqi Niu, Xiaoqing Qian, Wanwan Li and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in RSC advances, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.3field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 12 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 3 countries.

Dan ZouInstitute of Nano Biomedicine and Engineering, Shanghai Engineering Research Center for Intelligent Instrument for Diagnosis and Therapy, Department of Instrument Science & Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University 800 Dongchuan Road Shanghai 200240 China dxcui@sjtu.edu.cn.
Weijie WuState Key Lab of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University 800 Dongchuan Road Shanghai 200240 P. R. China wwli@sjtu.edu.cn.
Jingpu ZhangScientific Research Center, Shanghai Public Health Clinical Center, Fudan University 2901 Caolang Road Shanghai 201508 P. R. China.
Qiang MaTumor Department of Xintai People Hospital 1329 Xinfu Road Xintai Shandong Province China.
Sisi FanInstitute of Nano Biomedicine and Engineering, Shanghai Engineering Research Center for Intelligent Instrument for Diagnosis and Therapy, Department of Instrument Science & Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University 800 Dongchuan Road Shanghai 200240 China dxcui@sjtu.edu.cn.
Jin ChengInstitute of Nano Biomedicine and Engineering, Shanghai Engineering Research Center for Intelligent Instrument for Diagnosis and Therapy, Department of Instrument Science & Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University 800 Dongchuan Road Shanghai 200240 China dxcui@sjtu.edu.cn.
Dan LiState Key Lab of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University 800 Dongchuan Road Shanghai 200240 P. R. China wwli@sjtu.edu.cn.
Jiaqi NiuInstitute of Nano Biomedicine and Engineering, Shanghai Engineering Research Center for Intelligent Instrument for Diagnosis and Therapy, Department of Instrument Science & Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University 800 Dongchuan Road Shanghai 200240 China dxcui@sjtu.edu.cn.
Xiaoqing QianInstitute of Nano Biomedicine and Engineering, Shanghai Engineering Research Center for Intelligent Instrument for Diagnosis and Therapy, Department of Instrument Science & Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University 800 Dongchuan Road Shanghai 200240 China dxcui@sjtu.edu.cn.
Wanwan LiState Key Lab of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University 800 Dongchuan Road Shanghai 200240 P. R. China wwli@sjtu.edu.cn.ORCID https://orcid.org/0000-0003-3809-0737
Daxiang CuiInstitute of Nano Biomedicine and Engineering, Shanghai Engineering Research Center for Intelligent Instrument for Diagnosis and Therapy, Department of Instrument Science & Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University 800 Dongchuan Road Shanghai 200240 China dxcui@sjtu.edu.cn.ORCID https://orcid.org/0000-0003-4513-905X
Shanghai Jiao Tong University · CNFudan University · CNHebei Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Herein, we report a multiplex detection platform based on a suspension array with aggregation-induced emission luminogen (AIEgen) barcodes for simultaneous quantitative measurement of let-7b-5p, miR-16-5p and miR-19b-3p, which are associated with gastric cancer. A detection strategy by using a flow cytometer is proposed, which utilizes AIEgen-encoded microspheres to quantify the target miRNAs, and phycoerythrin as a fluorescence reporter on the detection probes to provide quantitative signals. This multiplex assay shows good specificity for recognizing single base mismatch, and possesses excellent sensitivity with limits of detection (LODs) ranging from 0.43 to 0.76 nM for the three miRNAs. The approach could be extended to the simultaneous detection of more target miRNAs by designing specific detection probes and increasing the number of fluorescence barcodes. We could foresee it holding great potential in future laboratory research and clinical applications due to its flexibility, strong multiplexed ability and good detection performance.

Identifiers

PMID35541422
PMCPMC9076168
OpenAlexW2994608485

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.