Evidence map›Paper›PMID 35541251›Full record

ArticleRSC advances2018

A ratiometric fluorescence assay for acetylcholinesterase activity and inhibitor screening based on supramolecular assembly induced monomer-excimer emission transition of a perylene probe.

Chunhua He, Huipeng Zhou, Ejaz Hussain, Yunyi Zhang, Niu Niu, Yunhui Li, Yuqin Ma, Cong Yu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in RSC advances, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Chunhua HeChangchun University of Science and Technology Weixing Road, No. 7989 Changchun 130022 P. R. China myq9393@sina.com.
Huipeng ZhouState Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences Changchun 130022 P. R. China congyu@ciac.ac.cn hpzhou@ciac.ac.cn.
Ejaz HussainState Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences Changchun 130022 P. R. China congyu@ciac.ac.cn hpzhou@ciac.ac.cn.
Yunyi ZhangState Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences Changchun 130022 P. R. China congyu@ciac.ac.cn hpzhou@ciac.ac.cn.
Niu NiuState Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences Changchun 130022 P. R. China congyu@ciac.ac.cn hpzhou@ciac.ac.cn.
Yunhui LiChangchun University of Science and Technology Weixing Road, No. 7989 Changchun 130022 P. R. China myq9393@sina.com.
Yuqin MaChangchun University of Science and Technology Weixing Road, No. 7989 Changchun 130022 P. R. China myq9393@sina.com.ORCID https://orcid.org/0000-0001-6286-1250
Cong YuState Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences Changchun 130022 P. R. China congyu@ciac.ac.cn hpzhou@ciac.ac.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A ratiometric fluorescence assay for acetylcholinesterase activity is established, which is based on controlled perylene probe assembly and monomer-excimer transition. In a buffer solution, a perylene probe with two negatively charged groups (PDI-DHA) mainly exists in monomeric form. In the presence of cationic lauroylcholine and lauric acid, PDI-DHA can form supramolecular assemblies and the perylene excimer emission can be observed. AChE can catalyze the hydrolysis of lauroylcholine to anionic lauric acid and choline. The hydrolysis process can trigger the breakdown of the supramolecular assemblies. The perylene excimer recovers to the monomeric form because of the de-aggregation of the probe. The excimer-monomer transition can be detected, and a ratiometric fluorescence assay for AChE activity and inhibitor screening is therefore established.

Identifiers

PMID35541251
PMCPMC9079431

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.