Evidence map›Paper›PMID 35540496›Full record

ArticleNature reviews. Methods primers2021

Recombineering and MAGE.

Timothy M Wannier, Peter N Ciaccia, Andrew D Ellington, Gabriel T Filsinger, Farren J Isaacs, Kamyab Javanmardi, Michaela A Jones, Aditya M Kunjapur, Akos Nyerges, Csaba Pal and 2 more

Abstract read
In one paragraph

Article in Nature reviews. Methods primers, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.

0numbers the graph read from it
0cells of the map it votes in
50citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

50 citing papers in PubMed.

  1. Review
  2. Oligonucleotide genetics forbioRxiv : the preprint server for biology · 2026
    Article
  3. Article
  4. Article
  5. Review
  6. Article
  7. Review
  8. Article
  9. Article
  10. Review
  11. Article
  12. Article
  13. The engineering toolbox of Parageobacillus thermoglucosidasius.Applied microbiology and biotechnology · 2025
    Review
  14. A diverse single-stranded DNA-annealing protein library enables efficient genome editing across bacterial phyla.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  15. Recent technology advancements in large-scale DNA assembly.Quantitative biology (Beijing, China) · 2025
    Article
  16. Review
  17. Bacterial live therapeutics for human diseases.Molecular systems biology · 2024
    Review
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Timothy M WannierDepartment of Genetics, Harvard Medical School, Boston, MA, USA.ORCID 0000-0002-4165-4751
Peter N CiacciaDepartment of Molecular, Cellular & Developmental Biology, Yale University, New Haven, CT, USA.ORCID 0000-0002-5745-6338
Andrew D EllingtonDepartment of Molecular Biosciences, College of Natural Sciences, University of Texas at Austin, Austin, TX, USA.ORCID 0000-0001-6246-5338
Gabriel T FilsingerWyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA.
Farren J IsaacsDepartment of Molecular, Cellular & Developmental Biology, Yale University, New Haven, CT, USA.
Kamyab JavanmardiDepartment of Molecular Biosciences, College of Natural Sciences, University of Texas at Austin, Austin, TX, USA.ORCID 0000-0002-6449-6709
Michaela A JonesDepartment of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE, USA.
Aditya M KunjapurDepartment of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE, USA.
Akos NyergesDepartment of Genetics, Harvard Medical School, Boston, MA, USA.ORCID 0000-0002-1581-490X
Csaba PalSynthetic and Systems Biology Unit, Institute of Biochemistry, Biological Research Centre, Szeged, Hungary.
Max G SchubertDepartment of Genetics, Harvard Medical School, Boston, MA, USA.
George M ChurchDepartment of Genetics, Harvard Medical School, Boston, MA, USA.

Funding

Chemistry-Biology Interface Predoctoral Training Grant 2024-2029T32GM133395 · NIGMS · UNIVERSITY OF DELAWARE · PI Catherine Leimkuhler Grimes · 2019 to 2026
$3.7M
Convergent Graduate Training in Engineering, Physics and BiologyT32EB019941 · NIBIB · YALE UNIVERSITY · PI O'HERN, COREY · 2016 to 2020
$998k
NIBIB NIH HHS T32 EB019941NIGMS NIH HHS T32 GM133395
6 · The paper itself

Abstract

Recombination-mediated genetic engineering, also known as recombineering, is the genomic incorporation of homologous single-stranded or double-stranded DNA into bacterial genomes. Recombineering and its derivative methods have radically improved genome engineering capabilities, perhaps none more so than multiplex automated genome engineering (MAGE). MAGE is representative of a set of highly multiplexed single-stranded DNA-mediated technologies. First described in

Identifiers

PMID35540496
PMCPMC9083505

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.