ArticleRSC advances2019
Analysis of amino acids in human tears by hydrophilic interaction liquid chromatography and quadrupole orbitrap mass spectrometry.
Article in RSC advances, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Development of an ultra-sensitive laser stimulated fluorescence system for simultaneous detection of amino acids.RSC advances · 2024Article
- A Look into Ocular Diseases: The Pivotal Role of Omics Sciences in Ophthalmology Research.ACS measurement science au · 2024Review
- Metabolomic changes in tear fluid following zinc biofortification in the BiZiFED nutritional study: a feasibility study.Frontiers in molecular biosciences · 2024Article
- Determination of Inorganic Ions in Parenteral Nutrition Solutions by Ion Chromatography.Molecules (Basel, Switzerland) · 2022Article
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Authors and funding
2 authors.
Funding
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Abstract
Amino acids in human tears play certain physiological roles and their determination is challenging due to complicated chemical properties. This study described a fast and sensitive method for the simultaneous determination of 15 amino acids (AAs) in tears by hydrophilic interaction liquid chromatography and quadrupole orbitrap mass spectrometry (HILIC-Q-Orbitrap-MS). Amino acids in tears were extracted by methanol, and then cleaned up with a solid phase extraction (SPE) cartridge. Chromatographic separation was performed on a 1.7 μm BEH Amide column within 8 min. Tear samples spiked with free AAs were tested in terms of linearity, sensitivity, repeatability, and recovery. Two stable isotope-labeled amino acids were used as internal standards to improve the method performance. Recoveries for all analytes ranged from 89 to 107%. Intra-day and inter-day precision, expressed as relative standard deviations, were all below 10%, and the method detection limits ranged from 0.02 μmol L
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Registered trials
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