ArticleRSC advances2019
Metagenomic ene-reductases for the bioreduction of sterically challenging enones.
Article in RSC advances, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 24 citations in OpenAlex.
- Asymmetric synthesis of alkyl fluoridesGreen chemistry : an international journal and green chemistry resource : GC · 2026Article
- The discovery of new metagenomic urethanases utilising a novel colorimetric assay for applications in the biodegradation of polyurethanes.Green chemistry : an international journal and green chemistry resource : GC · 2025Article
- The Impact of Metagenomics on Biocatalysis.Angewandte Chemie (International ed. in English) · 2024Review
- The Discovery of Imine Reductases and their Utilisation for the Synthesis of Tetrahydroisoquinolines.ChemCatChem · 2023Article
- Identification of a novel ene reductase fromRSC advances · 2022Article
- The Family Keeps on Growing: Four Novel Fungal OYEs Characterized.International journal of molecular sciences · 2022Article
- "A Study in Yellow": Investigations in the Stereoselectivity of Ene-Reductases.Chembiochem : a European journal of chemical biology · 2022Review
- Discovery of New Carbonyl Reductases Using Functional Metagenomics and Applications in Biocatalysis.Advanced synthesis & catalysis · 2021Article
- C=C-Ene-Reductases Reduce the C=N Bond of Oximes.ACS catalysis · 2020Article
- The identification and use of robust transaminases from a domestic drain metagenome.Green chemistry : an international journal and green chemistry resource : GC · 2019Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ene-reductases (ERs) of the Old Yellow Enzyme family catalyse asymmetric reduction of activated alkenes providing chiral products. They have become an important method in the synthetic chemists' toolbox offering a sustainable alternative to metal-catalysed asymmetric reduction. Development of new biocatalytic alkene reduction routes, however needs easy access to novel biocatalysts. A sequence-based functional metagenomic approach was used to identify novel ERs from a drain metagenome. From the ten putative ER enzymes initially identified, eight exhibited activities towards widely accepted mono-cyclic substrates with several of the ERs giving high reaction yields and stereoselectivities. Two highly performing enzymes that displayed excellent co-solvent tolerance were used for the stereoselective reduction of sterically challenging bicyclic enones where the reactions proceeded in high yields, which is unprecedented to date with wild-type ERs. On a preparative enzymatic scale, reductions of Hajos-Parish, Wieland-Miescher derivatives and a tricyclic ketone proceeded with good to excellent yields.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.