Evidence map›Paper›PMID 35538261›Full record

ArticleMikrochimica acta2022

Ultrasensitive fluorescence detection of microRNA through DNA-induced assembly of carbon dots on gold nanoparticles with no signal amplification strategy.

Mengyuan He, Ning Shang, Bo Zheng, Gege Yue, Xi Han, Xuebo Hu

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Mengyuan HeCollege of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang, 464000, China. myhe@xynu.edu.cn.ORCID http://orcid.org/0000-0002-8219-7921
Ning ShangCollege of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang, 464000, China.
Bo ZhengCollege of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang, 464000, China.
Gege YueCollege of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang, 464000, China.
Xi HanCollege of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang, 464000, China.
Xuebo HuCollege of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang, 464000, China.

Funding

National Natural Science Foundation of China 21804117Science and Technology Department of Henan Province 182102310703
6 · The paper itself

Abstract

An ultrasensitive fluorescence assay strategy on the basis of carbon dots (CDs) and cDNA-modified gold nanoparticles (AuNP-cDNA) was developed for the determination of microRNA-21 (miRNA-21) via internal filtering effect (IFE). Positively charged CDs (PEI-CDs), the fluorophores in IFE, were synthesized via a hydrothermal method using polyethyleneimine (PEI) as surface ligand. The maximum emission wavelength is located at 500 nm under the excitation of 410 nm. AuNPs, the absorbers, were modified with single-stranded DNA (cDNA), which is completely complementary to miRNA-21. The fluorescence of PEI-CDs is quenched due to the assembly of PEI-CDs and AuNPs-cDNA. In the presence of miRNA-21, the hybridization between miRNA-21 and cDNA causes the release of PEI-CDs and the recovery of fluorescence intensity.The fluorescence recovery degree is linearly correlated with the logarithm of miRNA-21 concentration in the range of 1-1000 fM. This method can be applied to determine miRNA-21 in real serum samples, and the detection results are in well agreement with those of qRT-PCR. The determination of miRNA-21 spiked into diluted human serum samples displays satisfactory recovery within the range 88.44-112.7%, which confirmed the reliability for miRNAs detection in real samples.

Indexed as

Metal NanoparticlesMicroRNAsQuantum DotsCarbonDNADNA, ComplementaryGoldHumansLimit of DetectionPolyethyleneimineReproducibility of ResultsCarbonDNADNA, ComplementaryGoldMicroRNAsPolyethyleneimineCarbon dotsFluorescence detectionGold nanoparticlesMicroRNAs

Identifiers

PMID35538261

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.