ArticleScientific reports2022
LincRNA#1 knockout alone does not affect polled phenotype in cattle heterozygous for the celtic POLLED allele.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed, 3 citations in OpenAlex.
- Genome-Wide Association Study and Whole-Genome Resequencing Reveal Key lincRNA and Candidate Gene for Polled Phenotype in Yak.Animals : an open access journal from MDPI · 2026Article
- The Development of Horns in Bovidae and the Genetic Mechanisms Underpinning This Process.Biology · 2025Review
- Establishment, characterization, and validation of novel porcine embryonic fibroblasts as a potential source for genetic modification.Frontiers in cell and developmental biology · 2022Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A long intergenic non-coding RNA (lincRNA#1) is overexpressed in the horn bud region of polled (hornless) bovine fetuses, suggesting a potential role in horn bud suppression. Genome editing was used to test whether the absence of this sequence was associated with the horned phenotype. Two gRNAs with high mutation efficiencies targeting the 5' and the 3' regions flanking the lincRNA#1 sequence were co-injected with Cas9 as ribonucleoprotein complexes into bovine zygotes (n = 121) 6 h post insemination. Of the resulting blastocysts (n = 31), 84% had the expected 3.7 kb deletion; of these embryos with the 3.7 kb deletions, 88% were biallelic knockouts. Thirty-nine presumptive edited 7-day blastocysts were transferred to 13 synchronized recipient cows resulting in ten pregnancies, five with embryos heterozygous for the dominant P
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