Evidence map›Paper›PMID 35537343›Full record

ArticlePoultry science2022

Rapid and visual detection of Mycoplasma synoviae by recombinase-aided amplification assay combined with a lateral flow dipstick.

Wenlong Xia, Ke Chen, Wensong Liu, Yan Yin, Qian Yao, Yu Ban, Yiwen Pu, Xingmin Zhan, Hongchun Bian, Shupei Yu and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Poultry science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Wenlong XiaYancheng Engineering Research Center of Animal Biologics, College of Marine and Biological Engineering, Yancheng Teachers University, Yancheng 224002, China.
Ke ChenYancheng Engineering Research Center of Animal Biologics, College of Marine and Biological Engineering, Yancheng Teachers University, Yancheng 224002, China.
Wensong LiuYancheng Engineering Research Center of Animal Biologics, College of Marine and Biological Engineering, Yancheng Teachers University, Yancheng 224002, China.
Yan YinYancheng Engineering Research Center of Animal Biologics, College of Marine and Biological Engineering, Yancheng Teachers University, Yancheng 224002, China.
Qian YaoYancheng Engineering Research Center of Animal Biologics, College of Marine and Biological Engineering, Yancheng Teachers University, Yancheng 224002, China.
Yu BanYancheng Engineering Research Center of Animal Biologics, College of Marine and Biological Engineering, Yancheng Teachers University, Yancheng 224002, China.
Yiwen PuYancheng Engineering Research Center of Animal Biologics, College of Marine and Biological Engineering, Yancheng Teachers University, Yancheng 224002, China.
Xingmin ZhanYancheng Engineering Research Center of Animal Biologics, College of Marine and Biological Engineering, Yancheng Teachers University, Yancheng 224002, China.
Hongchun BianYancheng Animal Husbandry and Veterinary Station, Yancheng 224001, China.
Shupei YuYancheng Animal Husbandry and Veterinary Station, Yancheng 224001, China.
Kunpeng HanYancheng Animal Husbandry and Veterinary Station, Yancheng 224001, China.
Ling YangYancheng Animal Husbandry and Veterinary Station, Yancheng 224001, China.
Huanli WangYancheng Engineering Research Center of Animal Biologics, College of Marine and Biological Engineering, Yancheng Teachers University, Yancheng 224002, China.
Zhongjun FanYancheng Engineering Research Center of Animal Biologics, College of Marine and Biological Engineering, Yancheng Teachers University, Yancheng 224002, China. Electronic address: fzj811@126.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mycoplasma synoviae (MS) is an important avian pathogen that has brought substantial economic losses to the global poultry industry. Fast and accurate diagnosis is one of the critical factors for the control of MS infection. This study established a simple, rapid and visual detection method for MS using a recombinase-aided amplification (RAA) combined with a lateral flow dipstick (LFD). The reaction temperature and time of the RAA-LFD assay were optimized after selecting the primers and probe, and the specificity and sensitivity rates were analyzed. The results showed that RAA could amplify the target gene in 20 min at a constant temperature of 38°C, and the amplification products could be visualized by LFD within 5 min. There was no cross-reaction with Mycoplasma gallisepticum (MG), Pasteurella multocida (P. multocida), Escherichia coli (E. coli), Newcastle disease virus (NDV), infectious bursal disease virus (IBDV), infectious bronchitis virus (IBV), and avian reovirus (ARV). Furthermore, the RAA-LFD assay exhibited high sensitivity with a detection limit of 10 copies/μL. A total of 128 clinical samples with suspected infection of MS were tested by RAA-LFD, PCR, and real-time fluorescence quantitative PCR (RFQ-PCR). The coincidence rate of the detection results was 95.3% between RAA-LFD and PCR, and 98.4% between RAA-LFD and RFQ-PCR. These results suggested that the RAA-LFD method established in the present study was easy to use and was associated with strong specificity and high sensitivity. This method was very suitable for the rapid detection of MS in clinical practice.

Indexed as

Mycoplasma synoviaeRecombinasesAnimalsChickensEscherichia coliNucleic Acid Amplification TechniquesSensitivity and SpecificityRecombinaseslateral flow dipstickMycoplasma synoviaePCRreal-time fluorescence quantitative PCRrecombinase-aided amplification

Identifiers

PMID35537343
PMCPMC9118145

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.