Evidence map›Paper›PMID 35536198›Full record

ArticleGenetics2022

BioKIT: a versatile toolkit for processing and analyzing diverse types of sequence data.

Jacob L Steenwyk, Thomas J Buida, Carla Gonçalves, Dayna C Goltz, Grace Morales, Matthew E Mead, Abigail L LaBella, Christina M Chavez, Jonathan E Schmitz, Maria Hadjifrangiskou and 2 more

Abstract read
In one paragraph

Article in Genetics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Patterns and drivers of genome-wide codon usage bias in the fungal order Sordariales.DNA research : an international journal for rapid publication of reports on genes and genomes · 2025
    Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Diverse signatures of convergent evolution in cacti-associated yeasts.bioRxiv : the preprint server for biology · 2023
    Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Jacob L SteenwykDepartment of Biological Sciences, Vanderbilt University, Nashville, TN 37235, USA.ORCID 0000-0002-8436-595X
Thomas J Buida9 City Place #312, Nashville, TN 37209, USA.ORCID 0000-0001-9367-6189
Carla GonçalvesDepartment of Biological Sciences, Vanderbilt University, Nashville, TN 37235, USA.ORCID 0000-0002-0420-4970
Dayna C Goltz2312 Elliston Place #510, Nashville, TN 37203, USA.
Grace MoralesDepartment of Pathology, Microbiology & Immunology, Center for Personalized Microbiology, Vanderbilt University Medical Center, Nashville, TN 37232, USA.ORCID 0000-0001-5454-9195
Matthew E MeadDepartment of Biological Sciences, Vanderbilt University, Nashville, TN 37235, USA.ORCID 0000-0001-9195-7585
Abigail L LaBellaDepartment of Biological Sciences, Vanderbilt University, Nashville, TN 37235, USA.ORCID 0000-0003-0068-6703
Christina M ChavezDepartment of Biological Sciences, Vanderbilt University, Nashville, TN 37235, USA.
Jonathan E SchmitzDepartment of Pathology, Microbiology & Immunology, Center for Personalized Microbiology, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
Maria HadjifrangiskouEvolutionary Studies Initiative, Vanderbilt University, Nashville, TN 37235, USA.
Yuanning LiDepartment of Biological Sciences, Vanderbilt University, Nashville, TN 37235, USA.ORCID 0000-0002-2206-5804
Antonis RokasDepartment of Biological Sciences, Vanderbilt University, Nashville, TN 37235, USA.ORCID 0000-0002-7248-6551

Funding

Deciphering the phenotypic and genomic traits that underlie the evolution of pathogenicity differences among Aspergillus fumigatus and its close relativesR01AI153356 · NIAID · VANDERBILT UNIVERSITY · PI Antonis Rokas · 2022 to 2026
$3.5M
Deciphering the phenotypic and genomic traits that underlie the evolution of pathogenicity differences among Aspergillus fumigatus and its close relativesR56AI146096 · NIAID · VANDERBILT UNIVERSITY · PI ROKAS, ANTONIS · 2020 to 2020
$592k
Howard Hughes Medical Institute through the James H. Gilliam FellowshipsNIAID NIH HHS R01 AI153356NIAID NIH HHS R56 AI146096
6 · The paper itself

Abstract

Bioinformatic analysis-such as genome assembly quality assessment, alignment summary statistics, relative synonymous codon usage, file format conversion, and processing and analysis-is integrated into diverse disciplines in the biological sciences. Several command-line pieces of software have been developed to conduct some of these individual analyses, but unified toolkits that conduct all these analyses are lacking. To address this gap, we introduce BioKIT, a versatile command line toolkit that has, upon publication, 42 functions, several of which were community-sourced, that conduct routine and novel processing and analysis of genome assemblies, multiple sequence alignments, coding sequences, sequencing data, and more. To demonstrate the utility of BioKIT, we conducted a comprehensive examination of relative synonymous codon usage across 171 fungal genomes that use alternative genetic codes, showed that the novel metric of gene-wise relative synonymous codon usage can accurately estimate gene-wise codon optimization, evaluated the quality and characteristics of 901 eukaryotic genome assemblies, and calculated alignment summary statistics for 10 phylogenomic data matrices. BioKIT will be helpful in facilitating and streamlining sequence analysis workflows. BioKIT is freely available under the MIT license from GitHub (https://github.com/JLSteenwyk/BioKIT), PyPi (https://pypi.org/project/jlsteenwyk-biokit/), and the Anaconda Cloud (https://anaconda.org/jlsteenwyk/jlsteenwyk-biokit). Documentation, user tutorials, and instructions for requesting new features are available online (https://jlsteenwyk.com/BioKIT).

Indexed as

Computational BiologySoftwareCodonSequence AlignmentSequence Analysis, DNACodonbioinformaticscodongenetic codegene-wise relative synonymous codon usagegenome assembly qualitymultiple sequence alignment

Identifiers

PMID35536198
PMCPMC9252278

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.