ArticleeLife2022
Single-cell monitoring of dry mass and dry mass density reveals exocytosis of cellular dry contents in mitosis.
Article in eLife, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 48 citations in OpenAlex.
- Inertial sensing of water content in tumor spheroids.Science advances · 2026Article
- Stochasticity in mammalian cell growth rates drives cell-to-cell variability independently of cell size and divisions.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
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- Diverse biophysical and molecular mechanisms drive phytoplankton sinking in response to starvation.PLoS biology · 2025Article
- High-throughput single-cell density measurements enable dynamic profiling of immune cell and drug response from patient samples.Nature biomedical engineering · 2025Article
- Biophysical and molecular mechanisms responsible for phytoplankton sinking in response to starvation.bioRxiv : the preprint server for biology · 2025Article
- Diverse roles of stress-responsive RNP granules in oogenesis and infertility.Biology of reproduction · 2025Review
- Plasma membrane folding enables constant surface area-to-volume ratio in growing mammalian cells.Current biology : CB · 2025Article
- Plasma membrane folding enables constant surface area-to-volume ratio in growing mammalian cells.bioRxiv : the preprint server for biology · 2025Article
- Cyanobacteria newly isolated from marine volcanic seeps display rapid sinking and robust, high-density growth.Applied and environmental microbiology · 2024Article
- Eukaryotic cell size regulation and its implications for cellular function and dysfunction.Physiological reviews · 2024Review
- Cell-intrinsic mechanical regulation of plasma membrane accumulation at the cytokinetic furrow.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Cell size, density, and nutrient dependency of unicellular algal gravitational sinking velocities.Science advances · 2024Article
- DHM/SERS reveals cellular morphology and molecular changes during iPSCs-derived activation of astrocytes.Biomedical optics express · 2024Article
- Cell intrinsic mechanical regulation of plasma membrane accumulation at the cytokinetic furrow.bioRxiv : the preprint server for biology · 2024Article
- Influence of the Age of Free-Living Amoeba Cysts on Their Vertical Distribution in a Water Column.Microorganisms · 2024Article
- Intracellular dry mass density increases under growth-induced pressure.Open research Europe · 2024Article
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- Epithelial tissue confinement inhibits cell growth and leads to volume-reducing divisions.Developmental cell · 2023Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Cell mass and composition change with cell cycle progression. Our previous work characterized buoyant mass dynamics in mitosis (Miettinen et al., 2019), but how dry mass and cell composition change in mitosis has remained unclear. To better understand mitotic cell growth and compositional changes, we develop a single-cell approach for monitoring dry mass and the density of that dry mass every ~75 s with 1.3% and 0.3% measurement precision, respectively. We find that suspension grown mammalian cells lose dry mass and increase dry mass density following mitotic entry. These changes display large, non-genetic cell-to-cell variability, and the changes are reversed at metaphase-anaphase transition, after which dry mass continues accumulating. The change in dry mass density causes buoyant and dry mass to differ specifically in early mitosis, thus reconciling existing literature on mitotic cell growth. Mechanistically, cells in early mitosis increase lysosomal exocytosis, and inhibition of lysosomal exocytosis decreases the dry mass loss and dry mass density increase in mitosis. Overall, our work provides a new approach for monitoring single-cell dry mass and dry mass density, and reveals that mitosis is coupled to extensive exocytosis-mediated secretion of cellular contents.
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