ArticleiScience2022
Cas9-mediated gene editing in the black-legged tick,
Article in iScience, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
44 citing papers in PubMed, 82 citations in OpenAlex.
- Infectious transgenesis in arthropods.bioRxiv : the preprint server for biology · 2026Article
- Pax6 homologs are required for patterning both visual systems of the daddy-longlegs Phalangium opilio.Molecular biology and evolution · 2026Article
- CRISPR-Cas9 mediated knockout of the white gene in the bluetongue virus vector, Culicoides sonorensis (biting midge).Scientific reports · 2026Article
- Genome-wide CRISPR knockout cell screening platform for the disease vector tick speciesbioRxiv : the preprint server for biology · 2026Article
- Article
- Tick Extracellular Vesicles Alter Epidermal Keratinocyte Function.The Journal of investigative dermatology · 2026Article
- HUH-tagged Cas9 as a platform for efficient ssODN-mediated knock-in via embryo and adult injection in insects.Communications biology · 2026Article
- Unlocking the Black Box: The Molecular Dialogue Between ASFV and Its Tick Host.Pathogens (Basel, Switzerland) · 2026Review
- Hl48 modulates argonaute 2 to enhance RNA interference in ticks.Frontiers in cellular and infection microbiology · 2026Article
- Duplex PCR assay to determine sex and mating status of Ixodes scapularis (Acari: Ixodidae), vector of the Lyme disease pathogen.Journal of medical entomology · 2025Article
- Article
- The mosaicism of Cas-induced mutations and pleiotropic effects of scarlet gene in an emerging model system.Heredity · 2025Article
- Tick Control Strategies: Critical Insights into Chemical, Biological, Physical, and Integrated Approaches for Effective Hard Tick Management.Veterinary sciences · 2025Review
- SYNCAS based CRISPR-Cas9 gene editing in predatory mites, whiteflies and stinkbugs.Insect biochemistry and molecular biology · 2025Article
- The hypusine pathway inFrontiers in cellular and infection microbiology · 2025Article
- Adapting and Testing ReMOT Control for Expanded CRISPR-Era Genome Functions in Non-model Insects.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Anaplasmosis in the Amazon: diagnostic challenges, persistence, and control ofFrontiers in veterinary science · 2025Review
- The Tick Microbiome: The "Other Bacterial Players" in Tick Biocontrol.Microorganisms · 2024Review
- A multi-omics approach for understanding blood digestion dynamics in Ixodes scapularis and identification of anti-tick vaccine targets.Ticks and tick-borne diseases · 2024Article
- Interventions for the control of Crimean-Congo hemorrhagic fever and tick vectors.NPJ vaccines · 2024Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 3 institutions in 1 country.
Funding
Abstract
Despite their capacity to acquire and pass on an array of debilitating pathogens, research on ticks has lagged behind other arthropod vectors, such as mosquitoes, largely because of challenges in applying available genetic and molecular tools. CRISPR-Cas9 is transforming non-model organism research; however, successful gene editing has not yet been reported in ticks. Technical challenges for injecting tick embryos to attempt gene editing have further slowed research progress. Currently, no embryo injection protocol exists for any chelicerate species, including ticks. Herein, we report a successful embryo injection protocol for the black-legged tick,
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.