ArticleNature plants2022
Distinct mechanisms for genomic attachment of the 5' and 3' ends of Agrobacterium T-DNA in plants.
Article in Nature plants, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Arabidopsis DNA repair mutants can integrate Agrobacterium T-DNA into the plant genome.The New phytologist · 2026Article
- The Genetic Transformation Mechanism and Application of Plant Hairy Roots.Plants (Basel, Switzerland) · 2026Review
- Beyond Short Microhomologies: Mismatch-Compatible Pol θ-Mediated DNA Damage Repair.BioEssays : news and reviews in molecular, cellular and developmental biology · 2026Review
- Plant tolerance mechanisms to DNA-damaging UV stress.Journal of experimental botany · 2025Review
- The evolving landscape of precise DNA insertion in plants.Nature communications · 2025Review
- Microhomology-Mediated End-Joining Chronicles: Tracing the Evolutionary Footprints of Genome Protection.Annual review of cell and developmental biology · 2024Review
- Arabidopsis F-box proteins D5BF1 and D5BF2 negatively regulate Agrobacterium-mediated transformation and tumorigenesis.Molecular plant pathology · 2024Article
- Genetic dissection of mutagenic repair and T-DNA capture at CRISPR-induced DNA breaks inPNAS nexus · 2024Article
- Genomic consequences associated with Agrobacterium-mediated transformation of plants.The Plant journal : for cell and molecular biology · 2024Review
- Characterization of integration sites and transfer DNA structures in Agrobacterium-mediated transgenic events of maize inbred B104.G3 (Bethesda, Md.) · 2023Article
- Regulation of gene editing using T-DNA concatenation.Nature plants · 2023Article
- The power of repetition.Nature plants · 2023Article
- Transformation and regeneration of DNA polymerase Θ mutant rice plants.Plant direct · 2023Article
- CRISPR/Cas9 and Agrobacterium tumefaciens virulence proteins synergistically increase efficiency of precise genome editing via homology directed repair in plants.Journal of experimental botany · 2023Article
- Effect of a suitable treatment period on the genetic transformation efficiency of the plant leaf disc method.Plant methods · 2023Article
- Identification of T-DNA structure and insertion site in transgenic crops using targeted capture sequencing.Frontiers in plant science · 2023Article
- Genetic modification, intercellular communication, and epigenetic regulation in plants: An outlook.Biochemical and biophysical research communications · 2022Review
- Genetic factors governing bacterial virulence and host plant susceptibility duringAdvances in genetics · 2022Review
Corrections and comments
- Commented on byMaking it stick.2022
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Agrobacterium tumefaciens, a pathogenic bacterium capable of transforming plants through horizontal gene transfer, is nowadays the preferred vector for plant genetic engineering. The vehicle for transfer is the T-strand, a single-stranded DNA molecule bound by the bacterial protein VirD2, which guides the T-DNA into the plant's nucleus where it integrates. How VirD2 is removed from T-DNA, and which mechanism acts to attach the liberated end to the plant genome is currently unknown. Here, using newly developed technology that yields hundreds of T-DNA integrations in somatic tissue of Arabidopsis thaliana, we uncover two redundant mechanisms for the genomic capture of the T-DNA 5' end. Different from capture of the 3' end of the T-DNA, which is the exclusive action of polymerase theta-mediated end joining (TMEJ), 5' attachment is accomplished either by TMEJ or by canonical non-homologous end joining (cNHEJ). We further find that TMEJ needs MRE11, whereas cNHEJ requires TDP2 to remove the 5' end-blocking protein VirD2. As a consequence, T-DNA integration is severely impaired in plants deficient for both MRE11 and TDP2 (or other cNHEJ factors). In support of MRE11 and cNHEJ specifically acting on the 5' end, we demonstrate rescue of the integration defect of double-deficient plants by using T-DNAs that are capable of forming telomeres upon 3' capture. Our study provides a mechanistic model for how Agrobacterium exploits the plant's own DNA repair machineries to transform it.
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