ArticleCommunications biology2022
Optimized single-step optical clearing solution for 3D volume imaging of biological structures.
Article in Communications biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- SynClear: A one-step synchronous clearing and labeling strategy for multiscale 3D brain mapping.Materials today. Bio · 2026Article
- 3D imaging with enhanced transparency, signal-to-background ratios, and antigen detection using HyPer-3D.Cell reports methods · 2026Article
- Article
- Optimizing tissue clearing methods for improved imaging of whole-mount retinas and optic nerves.Journal of neuroscience methods · 2025Article
- PRIAMOS: A technique for mixing embedding media for freely adjusting pH value and refractive index (RI) for optical clearing (OC) of whole tissue samples.Journal of microscopy · 2025Article
- Rapid and Solvent-Free, 2-hydroxyethyl Methacrylate (HEMA)-Acrylamide (AAm) Copolymer-Based Optical Clearing of Tissue for Fluorescent Imaging.Bio-protocol · 2025Article
- Rapid sonication-assisted whole tissue clearing and immunostaining.Scientific reports · 2025Article
- A novel protein-preserving passive tissue clearing approach using sodium cholate and urea for whole-organ imaging.Experimental & molecular medicine · 2025Article
- Three-dimensional assessments are necessary to determine the true, spatially resolved composition of tissues.Cell reports methods · 2025Article
- Application of HEMA-AAm copolymer to achieve faster optical tissue transparency for 2D/3D fluorescence imaging.Scientific reports · 2025Article
- 3D Histology visualizing hypoxia-induced upregulation of N-terminal cysteine using de novo fluorophore generation.Redox biology · 2025Article
- Comparison of Light-Sheet Fluorescence Microscopy and Fast-Confocal Microscopy for Three-Dimensional Imaging of Cleared Mouse Brain.Methods and protocols · 2023Article
- The life and times of endogenous opioid peptides: Updated understanding of synthesis, spatiotemporal dynamics, and the clinical impact in alcohol use disorder.Neuropharmacology · 2023Review
- Spatio-Temporal Dynamics of Diffusion-Associated Deformations of Biological Tissues and Polyacrylamide Gels Observed with Optical Coherence Elastography.Materials (Basel, Switzerland) · 2023Article
- Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Various optical clearing approaches have been introduced to meet the growing demand for 3D volume imaging of biological structures. Each has its own strengths but still suffers from low transparency, long incubation time, processing complexity, tissue deformation, or fluorescence quenching, and a single solution that best satisfies all aspects has yet been developed. Here, we develop OptiMuS, an optimized single-step solution that overcomes the shortcomings of the existing aqueous-based clearing methods and that provides the best performance in terms of transparency, clearing rate, and size retention. OptiMuS achieves rapid and high transparency of brain tissues and other intact organs while preserving the size and fluorescent signal of the tissues. Moreover, OptiMuS is compatible with the use of lipophilic dyes, revealing DiI-labeled vascular structures of the whole brain, kidney, spleen, and intestine, and is also applied to 3D quantitative and comparative analysis of DiI-labeled vascular structures of glomeruli turfs in normal and diseased kidneys. Together, OptiMuS provides a single-step solution for simple, fast, and versatile optical clearing method to obtain high tissue transparency with minimum structural changes and is widely applicable for 3D imaging of various whole biological structures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.