ArticleScientific reports2022
Optimising the photothrombotic model of stroke in the C57BI/6 and FVB/N strains of mouse.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 33 citations in OpenAlex.
- Inducing and monitoring photothrombotic stroke in anesthetic neuroprotection-free mice using functional photoacoustic microscopy.Neurophotonics · 2026Article
- Hemodynamic and Electrophysiological Progression of the Rose Bengal Photothrombotic Stroke Model in Mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026Article
- Sinensetin attenuates post-stroke depression via dual modulation of TLR4/NF-κB-NRF2/GPX4 pathways.Scientific reports · 2026Article
- A minimally invasive, scalable and reproducible neonatal rat model of severe focal brain injury.Brain communications · 2026Article
- Characterisation of the axon initial segment and intrinsic excitability in the sub-acute phase post-ischaemic stroke.Brain communications · 2026Article
- Article
- Behavioral tests to assess short- and long-lasting sensorimotor deficits following transient focal cerebral ischemia in rodents.Pharmacological reports : PR · 2025Review
- Catecholamine-Induced Inflammasome Activation in the Heart Following Photothrombotic Stroke.Translational stroke research · 2025Article
- Delivery of Angiogenic Therapy from Flowable Hyaluronic Acid Porous Scaffolds Results in Functional Improvement without Anti-Inflammatory Agents.Advanced functional materials · 2025Article
- Manual assessment of cylinder rearing behavior is more sensitive than automated gait evaluations in young, male mice post-stroke of the forepaw somatosensory cortex.Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association · 2025Article
- Drug screening for ischemic stroke using larvae and adult zebrafish model: a review.Laboratory animal research · 2025Review
- Exercise preconditioning alleviates photothrombotic ischemic stroke in mice by orchestrating neutrophils.Frontiers in physiology · 2025Article
- Suppressing the Inflammatory Prostaglandin Signaling after Thrombotic Stroke Ameliorates Ischemic Brain Injury and Facilitates Poststroke Recovery.ACS pharmacology & translational science · 2024Article
- TRPC Channels Activated by G Protein-Coupled Receptors Drive CaTranslational stroke research · 2024Article
- Hi1a Improves Sensorimotor Deficit following Endothelin-1-Induced Stroke in Rats but Does Not Improve Functional Outcomes following Filament-Induced Stroke in Mice.ACS pharmacology & translational science · 2024Article
- The Photothrombotic Model of Ischemic Stroke.Methods in molecular biology (Clifton, N.J.) · 2024Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The photothrombotic stroke model relies on the interaction between photosensitive-dye and light for clot formation. Interestingly, the relationship between the length of light exposure and stroke-outcome has never been examined. This model has yet to be established in the FVB/N strain, even though stroke-outcomes are strain-specific. Therefore, this study aimed to examine the effect of different lengths of light exposure in two strains of mice on photothrombotic stroke. Male FVB/N and C57Bl/6 mice were subjected to stroke using 15, 18, or 20-min light exposure. Mice underwent functional testing for up to 7 days. Infarct volume was assessed with thionin staining, and cellular responses to injury analysed via immunofluorescence at 7-days post-stroke. Blood brain barrier (BBB) breakdown was assessed using Evans blue dye at 4.5-h post-stroke. Increasing light exposure from 15 to 20-min increased infarct volume but not functional deficit. Interestingly, there were strain-specific differences in functional outcomes, with FVB/N mice having less deficit on the hanging wire test than C57BI/6 after 15-min of light exposure. The opposite was seen in the adhesive removal test. There was no difference in the number of neurons, astrocytes, microglia, macrophages, and T cells between the strains, despite FVB/N mice demonstrating greater BBB breakdown and an enlarged spleen post-stroke. Increasing light exposure systematically increases infarct volume but does not worsen functional outcomes. FVB/N and C57Bl/6 mice exhibit subtle differences in functional outcomes post stroke, which highlights the need to choose tests which are appropriate for the mouse strain being used.
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