ArticleNature communications2022
Double-tap gene drive uses iterative genome targeting to help overcome resistance alleles.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 17 citations in OpenAlex.
- Search-and-remove genome editing allows selection of cells by DNA sequence.Nature communications · 2025Article
- Cas12f1 gene drives propagate efficiently in herpesviruses and induce minimal resistance.Genome biology · 2024Article
- Repeat mediated excision of gene drive elements for restoring wild-type populations.PLoS genetics · 2024Article
- Population dynamics in spatial suppression gene drive models and the effect of resistance, density dependence, and life history.bioRxiv : the preprint server for biology · 2024Article
- Repeat mediated excision of gene drive elements for restoring wild-type populations.bioRxiv : the preprint server for biology · 2023Article
- Recursive Editing improves homology-directed repair through retargeting of undesired outcomes.Nature communications · 2022Article
- Targeting double-strand break indel byproducts with secondary guide RNAs improves Cas9 HDR-mediated genome editing efficiencies.Nature communications · 2022Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Homing CRISPR gene drives could aid in curbing the spread of vector-borne diseases and controlling crop pest and invasive species populations due to an inheritance rate that surpasses Mendelian laws. However, this technology suffers from resistance alleles formed when the drive-induced DNA break is repaired by error-prone pathways, which creates mutations that disrupt the gRNA recognition sequence and prevent further gene-drive propagation. Here, we attempt to counteract this by encoding additional gRNAs that target the most commonly generated resistance alleles into the gene drive, allowing a second opportunity at gene-drive conversion. Our presented "double-tap" strategy improved drive efficiency by recycling resistance alleles. The double-tap drive also efficiently spreads in caged populations, outperforming the control drive. Overall, this double-tap strategy can be readily implemented in any CRISPR-based gene drive to improve performance, and similar approaches could benefit other systems suffering from low HDR frequencies, such as mammalian cells or mouse germline transformations.
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Registered trials
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