ArticleProceedings of the National Academy of Sciences of the United States of America2022
Plant ESCRT protein ALIX coordinates with retromer complex in regulating receptor-mediated sorting of soluble vacuolar proteins.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 27 citations in OpenAlex.
- Beyond parallel pathways: molecular integration and functional crosstalk between autophagy and endocytosis in plants.Protoplasma · 2026Review
- EhVps29 Has a Role in the Location of the Retromer Complex and the Function of Key Virulence Factors inMicroorganisms · 2026Article
- Post-Golgi Trafficking in Plant Cells.Sub-cellular biochemistry · 2026Review
- Cargo Sorting Zones in Plant Trans-Golgi Network.Sub-cellular biochemistry · 2026Review
- ESCRTs in plant abiotic stresses.Essays in biochemistry · 2025Review
- Retrieval from vacuolar and endosomal compartments underpinning the neofunctionalization of SNARE in plants.Nature plants · 2025Article
- Vacuolar sorting receptors coordinate lytic vacuolar and autophagic transport for plant effector-triggered immunity.Nature plants · 2025Article
- Retromer protein VPS29 plays a crucial and positive role in the sumoylation system mediated by E3 SUMO ligase SIZ1.The Plant journal : for cell and molecular biology · 2025Article
- Contrasting Retromer with a Newly Described Retriever inPlants (Basel, Switzerland) · 2024Review
- Impact of acute heat stress on mitochondrial function, ultrastructure and cardiolipin distribution inaBIOTECH · 2024Article
- Arabidopsis CaLB1 undergoes phase separation with the ESCRT protein ALIX and modulates autophagosome maturation.Nature communications · 2024Article
- Arabidopsis retromer subunit AtVPS29 is involved in SLY1-mediated gibberellin signaling.Plant cell reports · 2024Article
- Vacuum-Based Agroinfiltration for Transient Gene Expression in Arabidopsis Seedlings.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Genetic Screening of Factors in the Plant Protein Secretion.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Advances in the Therapeutic Applications of Plant-Derived Exosomes in the Treatment of Inflammatory Diseases.Biomedicines · 2023Review
- A plant-unique protein BLISTER coordinates with core retromer to modulate endosomal sorting of plasma membrane and vacuolar proteins.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- EGFR trafficking: effect of dimerization, dynamics, and mutation.Frontiers in oncology · 2023Review
- RAB7 GTPases as coordinators of plant endomembrane traffic.Frontiers in plant science · 2023Review
- Diversity of retromer-mediated vesicular trafficking pathways in plants.Frontiers in plant science · 2023Review
- An unexpected function for an ESCRT protein.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
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Authors and funding
11 authors at 7 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vacuolar proteins play essential roles in plant physiology and development, but the factors and the machinery regulating their vesicle trafficking through the endomembrane compartments remain largely unknown. We and others have recently identified an evolutionarily conserved plant endosomal sorting complex required for transport (ESCRT)-associated protein apoptosis-linked gene-2 interacting protein X (ALIX), which plays canonical functions in the biogenesis of the multivesicular body/prevacuolar compartment (MVB/PVC) and in the sorting of ubiquitinated membrane proteins. In this study, we elucidate the roles and underlying mechanism of ALIX in regulating vacuolar transport of soluble proteins, beyond its conventional ESCRT function in eukaryotic cells. We show that ALIX colocalizes and physically interacts with the retromer core subunits Vps26 and Vps29 in planta. Moreover, double-mutant analysis reveals the genetic interaction of ALIX with Vps26 and Vps29 for regulating trafficking of soluble vacuolar proteins. Interestingly, depletion of ALIX perturbs membrane recruitment of Vps26 and Vps29 and alters the endosomal localization of vacuolar sorting receptors (VSRs). Taken together, ALIX functions as a unique retromer core subcomplex regulator by orchestrating receptor-mediated vacuolar sorting of soluble proteins.
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