ArticleMicrobial biotechnology2022
Enhancing fluxes through the mevalonate pathway in Saccharomyces cerevisiae by engineering the HMGR and β-alanine metabolism.
Article in Microbial biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 53 citations in OpenAlex.
- Snapshot at Saccharomycopsis fibuligera metabolism for industrial terpenoid production and bioengineering.BMC microbiology · 2026Article
- The Mevalonate Pathway: Innovations, Applications, and Challenges in Biotechnology with Emphasis on Fungal Biology.Journal of fungi (Basel, Switzerland) · 2026Review
- Combining multiple metabolic strategies for efficient production of longifolene inSynthetic and systems biotechnology · 2026Article
- Gold nanoparticle-mediated metabolic engineering in Trichoderma longibrachiatum MD33 unveils a hybrid terpenoid-alkaloid pathway for enhanced dendrobine biosynthesis.Microbial cell factories · 2026Article
- Centromeric Sequences in Ogataea polymorpha Genome Enable Development of Stable Multigene Expression Plasmid Tools.Microbial biotechnology · 2025Article
- Metabolic Engineering of Yeasts for the Production of the Triterpene Squalene: Current Status and Future Prospective.Microorganisms · 2025Review
- Engineering the oleaginous yeast Yarrowia lipolytica for co-production of phenolic monoterpenes thymol and carvacrol.Microbial cell factories · 2025Article
- Overexpression of tRNA mFrontiers in microbiology · 2025Article
- One-Pot Synthesis of β-Alanine from Fumaric Acid via an Efficient Dual-Enzyme Cascade Biotransformation.Biomolecules · 2024Article
- Integrative Transcriptomic and Target Metabolite Analysis as a New Tool for Designing Metabolic Engineering in Yeast.Biomolecules · 2024Article
- Microbial production of 5-Applied and environmental microbiology · 2024Article
- Microbial Squalene: A Sustainable Alternative for the Cosmetics and Pharmaceutical Industry - A Review.Engineering in life sciences · 2024Review
- Biological Properties of Sandalwood Oil and Microbial Synthesis of Its Major Sesquiterpenoids.Biomolecules · 2024Review
- Engineering Yeast Peroxisomes for α-Bisabolene Production from Sole Methanol with the Aid of Proteomic Analysis.JACS Au · 2024Article
- MeJA-induced hairy roots inFrontiers in plant science · 2024Article
- Miltiradiene Production by Cytoplasmic Metabolic Engineering inMetabolites · 2023Article
- Switching carbon metabolic flux for enhancing the production of sesquiterpene-based high-density biofuel precursor in Saccharomyces cerevisiae.Biotechnology for biofuels and bioproducts · 2023Article
- Regulation of Cat8 in energy metabolic balance and glucose tolerance in Saccharomyces cerevisiae.Applied microbiology and biotechnology · 2023Article
- PhiReX 2.0: A Programmable and Red Light-Regulated CRISPR-dCas9 System for the Activation of Endogenous Genes inACS synthetic biology · 2023Article
- Cofactor Engineering for Efficient Production of α-Farnesene by Rational Modification of NADPH and ATP Regeneration Pathway inInternational journal of molecular sciences · 2023Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mevalonate (MVA) pathway is the core for terpene and sterol biosynthesis, whose metabolic flux influences the synthesis efficiency of such compounds. Saccharomyces cerevisiae is an attractive chassis for the native active MVA pathway. Here, the truncated form of Enterococcus faecalis MvaE with only 3-Hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) activity was found to be the most effective enzyme for MVA pathway flux using squalene as the metabolic marker, resulting in 431-fold and 9-fold increases of squalene content in haploid and industrial yeast strains respectively. Furthermore, a positive correlation between MVA metabolic flux and β-alanine metabolic activity was found based on a metabolomic analysis. An industrial strain SQ3-4 with high MVA metabolic flux was constructed by combined engineering HMGR activity, NADPH regeneration, cytosolic acetyl-CoA supply and β-alanine metabolism. The strain was further evaluated as the chassis for terpenoids production. Strain SQ3-4-CPS generated from expressing β-caryophyllene synthase in SQ3-4 produced 11.86 ± 0.09 mg l
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