A spherical poly(acrylic acid) brush-enzyme block with high catalytic capacity for signal amplification in digital biological assays.

Yibei Wang, Zehang Gao, Jingwei Yi, Hongbo Zhou, Xiaoxia Fang, Hong Xu, Jianlong Zhao, Hongchen Gu

Abstract read

In one paragraph

Article in RSC advances, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yibei WangSchool of Biomedical Engineering, Med-X Research Institute, Shanghai Jiao Tong University Shanghai 200030 PR China xuhong@sjtu.edu.cn hcgu@sjtu.edu.cn.

Zehang GaoShanghai Institute of Microsystem and Information Technology, Chinese Academy Science Shanghai 200030 PR China.

Jingwei YiSchool of Biomedical Engineering, Med-X Research Institute, Shanghai Jiao Tong University Shanghai 200030 PR China xuhong@sjtu.edu.cn hcgu@sjtu.edu.cn.

Hongbo ZhouShanghai Institute of Microsystem and Information Technology, Chinese Academy Science Shanghai 200030 PR China.

Xiaoxia FangSchool of Biomedical Engineering, Med-X Research Institute, Shanghai Jiao Tong University Shanghai 200030 PR China xuhong@sjtu.edu.cn hcgu@sjtu.edu.cn.

Hong XuSchool of Biomedical Engineering, Med-X Research Institute, Shanghai Jiao Tong University Shanghai 200030 PR China xuhong@sjtu.edu.cn hcgu@sjtu.edu.cn.ORCID https://orcid.org/0000-0002-2787-5806

Jianlong ZhaoShanghai Institute of Microsystem and Information Technology, Chinese Academy Science Shanghai 200030 PR China.

Hongchen GuSchool of Biomedical Engineering, Med-X Research Institute, Shanghai Jiao Tong University Shanghai 200030 PR China xuhong@sjtu.edu.cn hcgu@sjtu.edu.cn.ORCID https://orcid.org/0000-0001-8009-2459

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Ultrasensitive determination of some ultra-low abundance biological molecules closely related to diseases is currently a wide concern and urgent issue to be addressed. Here, a spherical poly(acrylic acid)-alkaline phosphatase (SP-AKP) signal amplification block using spherical poly(acrylic acid) brush nanoparticles (SP) as the immobilized carriers was designed and synthesized optimally first. The results show that a single SP-AKP with high enzyme binding capacity and high catalytic ability (up to about 4800 effective free AKP per SP-AKP) has much greater fluorescence signal amplification ability than a single free AKP or SiO

Identifiers

PMID35530629

DOI10.1039/c9ra03404h

PMCPMC9069456

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.