ArticleRSC advances2019
Impact of deoxynivalenol and kaempferol on expression of tight junction proteins at different stages of Caco-2 cell proliferation and differentiation.
Article in RSC advances, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- Impacts of trichothecene mycotoxins on human colonic epithelial cells: molecular mechanisms and signaling pathways.Journal of advanced research · 2026Review
- Phenolic Compounds of Grape Pomace Skin Released During SHIME Colonic Fermentation Shape the Transcriptomic Profile of Tight Junctions, Improving the Barrier Properties in Caco-2 Cells.International journal of molecular sciences · 2026Article
- Comprehensive Characterization of Bioactive and Undesirable Compounds in Mezcal-Derived Vinasse for Potential Circular Applications.Foods (Basel, Switzerland) · 2026Article
- Interface-engineered Caco-2 cell culture on a collagen-coated liquid-liquid interface in a microfluidic device.Beilstein journal of nanotechnology · 2026Article
- Kaempferol in inflammatory bowel disease: regulating intestinal epithelial-fibroblast crosstalk.Frontiers in pharmacology · 2026Article
- Modulation of the Respiratory Epithelium Physiology by Flavonoids-Insights from 16HBEσcell Model.International journal of molecular sciences · 2024Article
- A Critical Review of Kaempferol in Intestinal Health and Diseases.Antioxidants (Basel, Switzerland) · 2023Review
- Traditional and emergingAnimal nutrition (Zhongguo xu mu shou yi xue hui) · 2023Article
- Article
- Exposure to the mycotoxin deoxynivalenol reduces the transport of conjugated bile acids by intestinal Caco-2 cells.Archives of toxicology · 2022Article
- Mycotoxins: Biotransformation and Bioavailability Assessment Using Caco-2 Cell Monolayer.Toxins · 2020Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The barrier function of intestinal tract is essential to gut health, and the expression of tight junction (TJ) proteins in human epithelial colorectal adenocarcinoma (Caco-2) cells, mimicking the intestinal stem cell proliferation and differentiation, was investigated after treatment by the mycotoxin of deoxynivalenol (DON) and phenolic compound of kaempferol (KAM). The results showed that DON (5 μM) significantly reduced the expression of claudin-4 while kaempferol (100 μM) increased the expression of claudin-3 during Caco-2 cell proliferation. After being cultured for 11 days, the DON treatment, unexpectedly, augmented the expression of claudin-4 with an increased TEER. For differentiated Caco-2 cells after a 21 day culture, both the TEER and claudin-4 levels were significantly reduced by DON while KAM pretreatment alleviated the damage caused by DON accompanying an increase of TEER, claudin-3, and ZO-1. Thus, kaempferol and DON differentially affected the expression of tight junction proteins at different stages of Caco-2 cell proliferation and differentiation, and an increase of the integrity of TJ after KAM pretreatment indicates KAM has the potential to protect the integrity of the barrier function of the intestinal epithelium for improved health.
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Registered trials
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