ArticleRSC advances2019
Medium optimization based on comparative metabolomic analysis of chicken embryo fibroblast DF-1 cells.
Article in RSC advances, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
4 citing papers in PubMed, 9 citations in OpenAlex.
- Exploring cost reduction strategies for serum free media development.NPJ science of food · 2024Review
- Serum metabolic alterations in chickens upon infectious bursal disease virus infection.BMC veterinary research · 2024Article
- Virus-like particles in poultry disease: an approach to effective and safe vaccination.Frontiers in veterinary science · 2024Review
- Regulation of the pyruvate metabolism node by monogene and polygene engineering of HEK-293 cells.RSC advances · 2019Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Chicken embryo fibroblast DF-1 cells are increasingly being used in the production of avian virus vaccines. However, the relatively low proliferative capacity does not meet the requirements of industrial production. In this study, we attempted to improve the proliferative capacity of DF-1 cells. The results of intracellular metabolomics showed that 28 types of metabolites could play roles in DF-1 cell growth based on the variance and timing analysis of intracellular metabolites from DF-1 cells grown in two media with distinct growth difference, DMEM/F12 (1 : 1) and DMEM. By examining the differences in the components in the two media, DOE was used to screen and optimize the growth medium for DF-1 cells. The maximum cell density was 40.72% higher, and the infectious bursal disease virus (IBDV) titer was 2.68 times higher, in the optimized medium than in the control. This study proposes a complete solution from metabolomics to media optimization.
Identifiers
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Registered trials
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