Evidence map›Paper›PMID 35528321›Full record

ArticleDevelopment & reproduction2022

Signal Transduction of C-Terminal Phosphorylation Regions for Equine Luteinizing Hormone/Chorionic Gonadotropin Receptor (eLH/CGR).

Munkhzaya Byambaragchaa, Hyo-Eun Joo, Sang-Gwon Kim, Yean-Ji Kim, Gyeong-Eun Park, Kwan-Sik Min

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Development & reproduction, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 53% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Munkhzaya ByambaragchaaInstitute of Genetic Engineering, Hankyong National University, Anseong 17579, Korea.ORCID https://orcid.org/0000-0002-0277-1816
Hyo-Eun JooDivision of Animal Science, School of Animal Life Convergence Sciences, Hankyong National University, Anseong 17579, Korea.ORCID https://orcid.org/0000-0002-8387-0428
Sang-Gwon KimDivision of Animal Science, School of Animal Life Convergence Sciences, Hankyong National University, Anseong 17579, Korea.ORCID https://orcid.org/0000-0003-3976-0931
Yean-Ji KimDivision of Animal Science, School of Animal Life Convergence Sciences, Hankyong National University, Anseong 17579, Korea.ORCID https://orcid.org/0000-0002-8411-2100
Gyeong-Eun ParkDivision of Animal Science, School of Animal Life Convergence Sciences, Hankyong National University, Anseong 17579, Korea.ORCID https://orcid.org/0000-0003-1740-3036
Kwan-Sik MinInstitute of Genetic Engineering, Hankyong National University, Anseong 17579, Korea.ORCID https://orcid.org/0000-0002-5451-3085
Hankyong National University · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study aimed to investigate the signal transduction of phosphorylation sites at the carboxyl (C)-terminal region of equine luteinizing hormone/chorionic gonadotropin receptor (eLH/ CGR). The eLH/CGR has a large extracellular domain of glycoprotein hormone receptors within the G protein-coupled receptors. We constructed a mutant (eLH/CGR-t656) of eLH/ CGR, in which the C-terminal cytoplasmic tail was truncated at the Phe656 residue, through polymerase chain reaction. The eLH/CGR-t656 removed 14 potential phosphorylation sites in the intracellular C-terminal region. The plasmids were transfected into Chinese hamster ovary (CHO)-K1 and PathHunter Parental cells expressing β-arrestin, and agonist-induced cAMP responsiveness was analyzed. In CHO-K1 cells, those expressing eLH/CGR-t656 were lower than those expressing eLH/CGR wild-type (eLH/CGR-wt). The EC

Indexed as

Chinese hamster ovary (CHO)-K1 cellsequine luteinizing hormone/chorionic gonadotropin receptor (eLH/CGR)PathHunter Parental cellsPhosphorylation sitesSignal transduction

Identifiers

PMID35528321
PMCPMC9042392
OpenAlexW4224297610

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.