ArticleMicrobial cell factories2022
Hot isopropanol quenching procedure for automated microtiter plate scale
Article in Microbial cell factories, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed.
- 13CFLUX - third-generation high-performance engine for isotopically (non)stationary 13C metabolic flux analysis.Bioinformatics (Oxford, England) · 2025Article
- Automated in vivo enzyme engineering accelerates biocatalyst optimization.Nature communications · 2024Review
- A High-Quality Genome-Scale Model forACS synthetic biology · 2023Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
backgroundCurrently, the generation of genetic diversity for microbial cell factories outpaces the screening of strain variants with omics-based phenotyping methods. Especially isotopic labeling experiments, which constitute techniques aimed at elucidating cellular phenotypes and supporting rational strain design by growing microorganisms on substrates enriched with heavy isotopes, suffer from comparably low throughput and the high cost of labeled substrates.
resultsWe present a miniaturized, parallelized, and automated approach to
conclusionsOur results show that a robotic liquid handler is sufficiently fast to generate informative isotopically transient labeling data. Furthermore, the amount of biomass obtained from a sub-milliliter cultivation in a microbioreactor is adequate for the detection of labeling patterns of free amino acids. Combining the innovations presented in this study, isotopically stationary and instationary automated labeling experiments can be conducted, thus fulfilling the prerequisites for
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Registered trials
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