Evidence map›Paper›PMID 35526203›Full record

ArticleBiotechnology letters2022

Knockout and functional analysis of BSSS-related genes in Acremonium chrysogenum by novel episomal expression vector containing Cas9 and AMA1.

Ling Liu, Zhen Chen, Xiwei Tian, Ju Chu

Abstract read
PubMed Publisher
In one paragraph

Article in Biotechnology letters, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 8 citations in OpenAlex.

  1. Engineering Industrial Strain ofAntibiotics (Basel, Switzerland) · 2026
    Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Ling LiuState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Zhen ChenState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Xiwei TianState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Ju ChuState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China. juchu@ecust.edu.cn.ORCID http://orcid.org/0000-0002-6406-1748
East China University of Science and Technology · CN

Funding

National Key Research Development Program of China No. 2019YFA0904800
6 · The paper itself

Abstract

objectiveThe target sorB gene, related to sorbicillinoid production, and the free expression element, AMA1, were used to verify the methodological approach in Acremonium chrysogenum.

resultCRISPR-Cas9 episomal expression system was used to introduce a point mutation into the sorB gene and the addition of sorB donor DNA achieved complete knockout of target genes. Four BSSS (yeast bud site selection system)-related genes, axl1, axl2, bud3, and bud4 were knocked out without impact on yield, dry weight, or pH. Relationships between morphology and stress tolerance in knockout strains were analyzed.

conclusionThe gene-editing system used in the current study exceeded 80% efficiency and arthrospores development was found to differ from that in wild-type strain.

Indexed as

AcremoniumSaccharomyces cerevisiae ProteinsCdc20 ProteinsCell Cycle ProteinsCephalosporinsCRISPR-Cas SystemsGene EditingGenes, FungalGTP-Binding ProteinsMembrane GlycoproteinsSaccharomyces cerevisiaeAma1 protein, S cerevisiaeAxl2 protein, S cerevisiaeBUD3 protein, S cerevisiaeBUD4 protein, S cerevisiaeCdc20 ProteinsCell Cycle ProteinsCephalosporinsGTP-Binding ProteinsMembrane GlycoproteinsSaccharomyces cerevisiae ProteinsAcremonium chrysogenumCephalosporin CCRISPR/Cas9Yeast bud site selection system

Identifiers

PMID35526203
OpenAlexW4229332485

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.