ArticleMicrobial cell factories2022
Toward more efficient ergothioneine production using the fungal ergothioneine biosynthetic pathway.
Article in Microbial cell factories, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
- Metabolic engineering ofSynthetic and systems biotechnology · 2026Article
- Metabolic engineering ofSynthetic and systems biotechnology · 2026Article
- Engineering Escherichia coli Nissle 1917 for the highly efficient biosynthesis of food-grade ergothioneine.Applied biochemistry and biotechnology · 2026Article
- Edible Fungi and Skin Redox Homeostasis: Bioactive Diversity, Processing, Oral Exposure, and Evidence Gaps.Antioxidants (Basel, Switzerland) · 2026Review
- Ergothioneine: Biosynthesis, Molecular Mechanisms, Physiological Function, and Role in Disease.MedComm · 2026Review
- Efficient Synthesis of Ergothioneine in Escherichia coli Through Identification of Bacterial-Type Egt1 and Self-Assemble of Pathway Enzymes.Biotechnology and bioengineering · 2026Article
- High-Level Production of Ergothioneine in Yarrowia lipolytica via Systematic Metabolic Engineering Strategies.Biotechnology and bioengineering · 2026Article
- Impact of DNA Extraction Strategies on Genomic and Bioinformatic Outcomes in Eight Selected Fungal Strains.Journal of fungi (Basel, Switzerland) · 2026Article
- Light controls gene functions through alternative splicing in fungi.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Enhanced production of ergothioneine in Aspergillus oryzae.Applied microbiology and biotechnology · 2025Article
- A Review of Novel Antioxidant Ergothioneine: Biosynthesis Pathways, Production, Function and Food Applications.Foods (Basel, Switzerland) · 2025Review
- Heterologous and High Production of Ergothioneine inMetabolites · 2025Article
- Ergothioneine biosynthesis: The present state and future prospect.Synthetic and systems biotechnology · 2025Review
- Article
- The Catalytic Mechanism of Key Enzymes Involved in the Synthesis of Ergothioneine inMolecules (Basel, Switzerland) · 2024Article
- Engineering non-conventional yeast Rhodotorula toruloides for ergothioneine production.Biotechnology for biofuels and bioproducts · 2024Article
- Structure of mycobacterial ergothioneine-biosynthesis C-S lyase EgtE.The Journal of biological chemistry · 2024Article
- A SingleInternational journal of molecular sciences · 2022Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundErgothioneine (ERG) is a potent histidine-derived antioxidant that confers health-promoting effects. Only certain bacteria and fungi can biosynthesize ERG, but the ERG productivity in natural producers is low. ERG overproduction through genetic engineering represents an efficient and cost-effective manufacturing strategy.
resultsHere, we showed that Trichoderma reesei can synthesize ERG during conidiogenesis and hyphal growth. Co-expression of two ERG biosynthesis genes (tregt1 and tregt2) from T. reesei enabled E. coli to generate 70.59 mg/L ERG at the shaking flask level after 48 h of whole-cell biocatalysis, whereas minor amounts of ERG were synthesized by the recombinant E. coli strain bearing only the tregt1 gene. By fed-batch fermentation, the extracellular ERG production reached 4.34 g/L after 143 h of cultivation in a 2-L jar fermenter, which is the highest level of ERG production reported thus far. Similarly, ERG synthesis also occurred in the E. coli strain engineered with the two well-characterized genes from N. crassa and the ERG productivity was up to 4.22 g/L after 143 h of cultivation under the above-mentioned conditions.
conclusionsOur results showed that the overproduction of ERG in E. coli could be achieved through two-enzymatic steps, demonstrating high efficiency of the fungal ERG biosynthetic pathway. Meanwhile, this work offers a more promising approach for the industrial production of ERG.
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