Evidence map›Paper›PMID 35525880›Full record

ArticleHepatology international2022

Tumor-matrix interaction induces phenotypic switching in liver cancer cells.

Ray-Hwang Yuan, Chia-Lang Hsu, Yu-Lin Jhuang, Yun-Ru Liu, Tsung-Han Hsieh, Yung-Ming Jeng

Abstract read
PubMed Publisher
In one paragraph

Article in Hepatology international, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.7field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Ray-Hwang Yuan *Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan.
Chia-Lang Hsu *Department of Medical Research, National Taiwan University Hospital, Taipei, Taiwan.
Yu-Lin JhuangGraduate Institute of Pathology, National Taiwan University, Taipei, Taiwan.
Yun-Ru LiuJoint Biobank Office of Human Research, Taipei Medical University, Taipei, Taiwan.
Tsung-Han HsiehJoint Biobank Office of Human Research, Taipei Medical University, Taipei, Taiwan.
Yung-Ming JengGraduate Institute of Pathology, National Taiwan University, Taipei, Taiwan. mrna0912@gmail.com.tw.
National Taiwan University Hospital · TWTaipei Medical University · TWNational Taiwan University · TW

Funding

Ministry of Science and Technology, Republic of China 108-2320-B-002 -059 -MY3Ministry of Science and Technology, Republic of China 109-2314-B-002 -082 -
6 · The paper itself

Abstract

backgroundIntrahepatic cholangiocarcinoma (ICC) is characterized by fibrous stroma and clinical behavior more aggressive than that of hepatocellular carcinoma (HCC). Scirrhous HCC is a subtype of HCC with fibrous stroma, frequently has partial cholangiocytic differentiation, and is more likely to have an aggressive behavior. This study explored the interaction of liver cancer cells with the extracellular matrix. METHODS AND

resultsLiver cancer cells grown on collagen 1-coated plates showed upregulation of cholangiocytic marker expression but downregulation of hepatocytic marker expression. Three-dimensional sphere culture and Boyden chamber assay showed enhanced invasion and migration ability in collagen 1-conditioned liver cancer cells. Interaction with collagen 1 reduced liver cancer cell proliferation. RNA sequencing showed that in the liver cancer cells, collagen 1 upregulated cell cycle inhibitor expression and cell-matrix interaction, tumor migration, and angiogenesis pathways, but downregulated liver metabolic function pathways. Cholangiocytic differentiation and invasiveness induced by collagen 1 was mediated by the mitogen-activated protein kinase (MAPK) pathway, which was regulated by cell-matrix interaction-induced Src activation. Analysis of the Cancer Genome Atlas cohort showed that collagen 1 induced and suppressed genes were highly enriched in ICC and HCC, respectively. In HCC samples, collagen 1-regulated genes were strongly coexpressed and correlated with COL1A1 expression.

conclusionsLiver cancer cell-matrix interaction induces cholangiocytic differentiation and switches liver cancer cells from a proliferative to an invasive phenotype through the Src/MAPK pathway, which may partly explain the differences in the behaviors of HCC and ICC.

Indexed as

Bile Duct NeoplasmsCarcinoma, HepatocellularCholangiocarcinomaLiver NeoplasmsBile Ducts, IntrahepaticCell Line, TumorCollagenHumansNeoplasm InvasivenessPhenotypeCollagenCholangiocarcinomaExtracellular matrixHepatocellular carcinomaInvasionMitogen-activated protein kinase

Identifiers

PMID35525880
OpenAlexW4229065473

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.