ArticleJournal of extracellular vesicles2022
The development of extracellular vesicle markers for the fungal phytopathogen Colletotrichum higginsianum.
Article in Journal of extracellular vesicles, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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Who cites it
23 citing papers in PubMed, 34 citations in OpenAlex.
- Extracellular Vesicles: Key Mediators of Bioactive Molecule Transport in Plant-Microbe Interactions.Molecular plant pathology · 2026Review
- Stage-Specific RNA Turnover Drives Small RNA Dynamics inPlant direct · 2026Article
- Environmental Regulation, Molecular Profiling, and Preliminary Functional Evaluation of Extracellular Vesicles fromFoods (Basel, Switzerland) · 2026Article
- Fungal Exosome-Like Nanoparticles from Huaier (Trametes robiniophila Murr.) Exhibit Antibreast Cancer Activity.Current developments in nutrition · 2026Article
- Isolation and proteomic analysis of intracellular vesicles from the potato late blight pathogen Phytophthora infestans.Scientific reports · 2026Article
- Plant-derived extracellular vesicles and nanoparticles: origins, functions, and applications.Frontiers in bioengineering and biotechnology · 2026Review
- Functional and proteomic characterization ofFrontiers in plant science · 2026Article
- Establishment of an Isolation System for Extracellular Vesicles ofJournal of fungi (Basel, Switzerland) · 2025Article
- Immunogold labeling of extracellular vesicles from the fungal pathogen Fusarium graminearum reveals the presence of the protein marker Sur7.Fungal biology and biotechnology · 2025Article
- Isolation and Characterization of Extracellular Vesicles From Ascochyta rabiei, a Necrotrophic Fungal Chickpea Pathogen.Proteomics · 2025Article
- Characterizing extracellular vesicles of human fungal pathogens.Nature microbiology · 2025Review
- Establishment of an experimental system to analyse extracellular vesicles during apoplastic fungal pathogenesis.Journal of extracellular biology · 2025Article
- Isolation, Characterization, and Proteomic Analysis of Crude and Purified Extracellular Vesicles Extracted fromPlants (Basel, Switzerland) · 2024Article
- Practical advice for extracellular vesicle isolation in plant-microbe interactions: Concerns, considerations, and conclusions.Journal of extracellular vesicles · 2024Article
- Fungal Extracellular Vesicle Proteins with Potential in Biological Interaction.Molecules (Basel, Switzerland) · 2024Review
- Extracellular Vesicles fromJournal of fungi (Basel, Switzerland) · 2024Article
- Extracellular vesicles from the mycoparasitic fungus Trichoderma harzianum.Antonie van Leeuwenhoek · 2024Article
- The Adaptation ofJournal of fungi (Basel, Switzerland) · 2023Article
- Extracellular vesicles: cross-organismal RNA trafficking in plants, microbes, and mammalian cells.Extracellular vesicles and circulating nucleic acids · 2023Article
- Extracellular Vesicles of the Plant PathogenJournal of fungi (Basel, Switzerland) · 2023Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 3 countries.
Funding
Abstract
Fungal phytopathogens secrete extracellular vesicles (EVs) associated with enzymes and phytotoxic metabolites. While these vesicles are thought to promote infection, defining the true contents and functions of fungal EVs, as well as suitable protein markers, is an ongoing process. To expand our understanding of fungal EVs and their possible roles during infection, we purified EVs from the hemibiotrophic phytopathogen Colletotrichum higginsianum, the causative agent of anthracnose disease in multiple plant species, including Arabidopsis thaliana. EVs were purified in large numbers from the supernatant of protoplasts but not the supernatant of intact mycelial cultures. We purified two separate populations of EVs, each associated with over 700 detected proteins, including proteins involved in vesicle transport, cell wall biogenesis and the synthesis of secondary metabolites. We selected two SNARE proteins (Snc1 and Sso2) and one 14-3-3 protein (Bmh1) as potential EV markers and generated transgenic strains expressing fluorescent fusions. Each marker was confirmed to be protected inside EVs. Fluorescence microscopy was used to examine the localization of each marker during infection on Arabidopsis leaves. These findings further our understanding of EVs in fungal phytopathogens and will help build an experimental system to study EV interkingdom communication between plants and fungi.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.