ArticleBiotechnology and bioengineering2022
Engineering Pseudomonas putida for improved utilization of syringyl aromatics.
Article in Biotechnology and bioengineering, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 23 citations in OpenAlex.
- Multi-strain analysis ofmSystems · 2026Article
- Unique metabolic traits ofJournal of bacteriology · 2026Review
- Multi-strain Analysis ofbioRxiv : the preprint server for biology · 2025Article
- Conversion and upgrading of syringate by Acinetobacter baylyi ADP1.Microbial cell factories · 2025Article
- Catabolic System of Syringic Acid, a Key Intermediate of Lignin-Derived Aromatic Compounds, via a Novel Linear Pathway inJournal of agricultural and food chemistry · 2025Article
- Screening of microbial consortium with high efficiency of lignin-degrading and its synergistic metabolic mechanism.Frontiers in microbiology · 2025Article
- Impact of fleQ Deficiency on Resource Allocation and Heterologous Gene Expression in Pseudomonas putida Across Various Growth Media.Microbial biotechnology · 2024Article
- Machine learning analysis of RB-TnSeq fitness data predicts functional gene modules inmSystems · 2024Article
- Application of functional genomics for domestication of novel non-model microbes.Journal of industrial microbiology & biotechnology · 2024Review
- Identification of a Phylogenetically Divergent Vanillate O-Demethylase fromMicroorganisms · 2022Article
- Engineering Pseudomonas putida for improved utilization of syringyl aromatics.Biotechnology and bioengineering · 2022Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Lignin is a largely untapped source for the bioproduction of value-added chemicals. Pseudomonas putida KT2440 has emerged as a strong candidate for bioprocessing of lignin feedstocks due to its resistance to several industrial solvents, broad metabolic capabilities, and genetic amenability. Here we demonstrate the engineering of P. putida for the ability to metabolize syringic acid, one of the major products that comes from the breakdown of the syringyl component of lignin. The rational design was first applied for the construction of strain Sy-1 by overexpressing a native vanillate demethylase. Subsequent adaptive laboratory evolution (ALE) led to the generation of mutations that achieved robust growth on syringic acid as a sole carbon source. The best mutant showed a 30% increase in the growth rate over the original engineered strain. Genomic sequencing revealed multiple mutations repeated in separate evolved replicates. Reverse engineering of mutations identified in agmR, gbdR, fleQ, and the intergenic region of gstB and yadG into the parental strain recaptured the improved growth of the evolved strains to varied extent. These findings thus reveal the ability of P. putida to utilize lignin more fully as a feedstock and make it a more economically viable chassis for chemical production.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.