ArticleNature neuroscience2022
Trans-Seq maps a selective mammalian retinotectal synapse instructed by Nephronectin.
Article in Nature neuroscience, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed, 94 citations in OpenAlex.
- Single-cell sequencing-guided discovery of narciclasine as a dual VCAM-1/ICAM-1 inhibitor for atherosclerosis management.Genes & diseases · 2027Article
- A novel mWGA-TYR PET reporter gene system for in vivo visualization of whole brain-level neural circuit.European journal of nuclear medicine and molecular imaging · 2026Article
- Distinct parafascicular neuronal ensembles mediate the sensory and affective dimensions of trigeminal neuralgia.The Journal of clinical investigation · 2026Article
- WTR: A Toolkit for Functional Anterograde Transsynaptic Circuit Mapping.Research square · 2026Article
- Temporally segregated functions for Gfi1 in the development and maintenance of direction selectivity in the vertebrate retina.Current biology : CB · 2026Article
- TRACR: an anterograde transneuronal tracing system for genetic access across synapses and longitudinal circuit analysis.bioRxiv : the preprint server for biology · 2026Article
- Parallel cholinergic circuit in oculomotor nucleus to control eye movements and REM sleep.Nature communications · 2026Article
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- CBLN2 promoter enables genetic access to wide-field neurons of the tree shrew superior colliculus.Cell reports methods · 2026Article
- Substance P in the lateral hypothalamic area regulates binge-like eating behaviors in mice.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Recovery of Retinal Terminal Fields after Traumatic Brain Injury: Evidence of Collateral Sprouting and Sexual Dimorphism.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026Article
- Synaptic control of retinal ganglion cell survival and axon regeneration.Molecular neurodegeneration · 2026Review
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- A bottom-up septal inhibitory circuit mediates anticipatory control of drinking.Nature neuroscience · 2025Article
- Hippocampal neural circuit mechanisms in Alzheimer's disease revealed by viral-genetic circuit mapping.Neurobiology of disease · 2025Review
- Co-Conservation of synaptic gene expression and circuitry in collicular neurons.Nature communications · 2025Article
- Article
- Modeling neurodegeneration in the retina and strategies for developing pan-neurodegenerative therapies.Molecular neurodegeneration · 2025Review
- Recovery of retinal terminal fields after traumatic brain injury: evidence of collateral sprouting and sexual dimorphism.bioRxiv : the preprint server for biology · 2025Article
6 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
15 authors at 3 institutions in 1 country.
Funding
Abstract
The mouse visual system serves as an accessible model to understand mammalian circuit wiring. Despite rich knowledge in retinal circuits, the long-range connectivity map from distinct retinal ganglion cell (RGC) types to diverse brain neuron types remains unknown. In this study, we developed an integrated approach, called Trans-Seq, to map RGCs to superior collicular (SC) circuits. Trans-Seq combines a fluorescent anterograde trans-synaptic tracer, consisting of codon-optimized wheat germ agglutinin fused to mCherry, with single-cell RNA sequencing. We used Trans-Seq to classify SC neuron types innervated by genetically defined RGC types and predicted a neuronal pair from αRGCs to Nephronectin-positive wide-field neurons (NPWFs). We validated this connection using genetic labeling, electrophysiology and retrograde tracing. We then used transcriptomic data from Trans-Seq to identify Nephronectin as a determinant for selective synaptic choice from αRGC to NPWFs via binding to Integrin α8β1. The Trans-Seq approach can be broadly applied for post-synaptic circuit discovery from genetically defined pre-synaptic neurons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.