Evidence map›Paper›PMID 35524124›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

A Strong Cation Exchange Chromatography Protocol for Examining N-Terminal Proteoforms.

Esperanza Fernández, Annelies Bogaert, Evy Timmerman, An Staes, Francis Impens, Kris Gevaert

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
3.0field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 4 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Esperanza FernándezVIB Center for Medical Biotechnology, Ghent, Belgium.
Annelies BogaertVIB Center for Medical Biotechnology, Ghent, Belgium.
Evy TimmermanVIB Center for Medical Biotechnology, Ghent, Belgium.
An StaesVIB Center for Medical Biotechnology, Ghent, Belgium.
Francis ImpensVIB Center for Medical Biotechnology, Ghent, Belgium.
Kris GevaertVIB Center for Medical Biotechnology, Ghent, Belgium. kris.gevaert@vib-ugent.be.
VIB-UGent Center for Medical Biotechnology · BEGhent University · BE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Especially in eukaryotes, the N-terminal acetylation status of a protein reveals translation initiation sites and substrate specificities and activities of N-terminal acetyltransferases (NATs). Here, we discuss a bottom-up proteomics protocol for the enrichment of N-terminal peptides via strong cation exchange chromatography. This protocol is based on depleting internal tryptic peptides from proteome digests through their retention on strong cation exchangers, leaving N-terminally acetylated/blocked peptides enriched among the nonretained peptides. As such, one can identify novel N-terminal proteoforms and quantify the degree of N-terminal protein acetylation.

Indexed as

ProteomeProteomicsAcetylationChromatographyPeptidesPeptidesProteomeN-terminal acetylationN-terminal acetyltransferasesN-terminomics

Identifiers

PMID35524124
OpenAlexW4285153799

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.