Heterologous Expression, Purification, and Characterization of Type II Polyketide Synthase Acyl Carrier Proteins.

Grayson S Hamrick, Casey H Londergan, Louise K Charkoudian

Open access · hybridAbstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

0.9field-weighted citation impact, top 27% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Grayson S HamrickDepartment of Chemistry, Haverford College, Haverford, PA, USA.

Casey H LonderganDepartment of Chemistry, Haverford College, Haverford, PA, USA.

Louise K CharkoudianDepartment of Chemistry, Haverford College, Haverford, PA, USA. lcharkou@haverford.edu.

Haverford College · US

Funding

Functional Hybrid Natural Product Synthases by Tracking Acyl Carrier Protein Binding and Conformational DynamicsR15GM120704 · NIGMS · HAVERFORD COLLEGE · PI CHARKOUDIAN, LOUISE KARINE · 2016 to 2024

$1.2M

NIGMS NIH HHS R15 GM120704

6 · The paper itself

Abstract

The enzymes that comprise type II polyketide synthases (PKSs) are powerful biocatalysts that, once well-understood and strategically applied, could enable cost-effective and sustainable access to a range of pharmaceutically relevant molecules. Progress toward this goal hinges on gaining ample access to materials for in vitro characterizations and structural analysis of the components of these synthases. A central component of PKSs is the acyl carrier protein (ACP), which serves as a hub during the biosynthesis of type II polyketides. Herein, we share methods for accessing type II PKS ACPs via heterologous expression in E. coli . We also share how the installation of reactive and site-specific spectroscopic probes can be leveraged to study the conformational dynamics and interactions of type II PKS ACPs.

Indexed as

Acyl Carrier ProteinPolyketide SynthasesEscherichia coliAcyl Carrier ProteinPolyketide SynthasesAcyl carrier proteinHeterologous expressionPolyaromatic polyketidePolyketide synthaseType II polyketide

Identifiers

PMID35524054

DOI10.1007/978-1-0716-2273-5_13

PMCPMC9373356

OpenAlexW4285189246

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.