ArticleScientific reports2022
An extensive description of the microbiological effects of silver diamine fluoride on dental biofilms using an oral in situ model.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 9 citations in OpenAlex.
- Silver diamine fluoride for non-operative restorative treatment and prevention of dental caries.Canadian journal of dental hygiene : CJDH = Journal canadien de l'hygiene dentaire : JCHD · 2026Review
- Article
- Effect of ammonia-free silver fluoride application on the resin-dentin interface subjected to an in situ cariogenic challenge.Clinical oral investigations · 2026Article
- Effectiveness of silver diamine fluoride in preventing and arresting dental caries in pediatric dentistry.Dental research journal · 2026Review
- Nanoplastics-mediated physiologic and genomic responses in pathogenic Escherichia coli O157:H7.Journal of nanobiotechnology · 2025Article
- Frontiers of Global Research Trend on Root Caries: A Bibliometric Analysis.International dental journal · 2024Review
- In Vitro Inhibitory Effect of Silver Diamine Fluoride Combined with Potassium Iodide against Mixed-Species Biofilm Formation on Human Root Dentin.Antibiotics (Basel, Switzerland) · 2024Article
- Unexpected sequel to the application of silver fluoride followed by stannous fluoride to an open carious lesion in a primary molar: A case report.Clinical and experimental dental research · 2024Article
- Effect of Silver Diamine Fluoride on Bacterial Biofilms-A Review including In Vitro and In Vivo Studies.Biomedicines · 2023Review
- Differential immune responses of 3D gingival and periodontal connective tissue equivalents to microbial colonization.Journal of tissue engineeringArticle
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Silver diamine fluoride (SDF) has been long studied in laboratories, and its clinical effectiveness in the treatment and prevention of root caries has been reported. In the present study, we assessed the microbiological effects of SDF on dental biofilms grown on demineralized dentin in situ. Specifically, demineralized bovine root dentin slabs used as biofilm substrates were treated with 38% SDF, and the biofilms formed after this treatment were analyzed via real-time PCR, DEAD/LIVE cell staining, and SEM. Next, the viable cell count was determined, and microbial profiles were compared using 16S rRNA gene sequencing. Untreated slabs were used as controls. We observed significant decreases in viable cell counts (p < 0.05), number of biofilm-forming cells (p < 0.01), biofilm thickness (p < 0.01), and high proportion of dead cells with SDF treatment (p < 0.01). The microcolonies in the SDF-treated biofilms showed less complexity, and only a limited number of genera were differentially abundant between the groups. Microbial diversity index comparisons showed no significant differences between the groups with respect to treatments days (p = 0.362). Thus, SDF negatively influenced dental biofilm growth on demineralized root dentin in situ; however, its antimicrobial action did not target a specific oral taxon.
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