ArticlePLoS genetics2022
Dnmt1a is essential for gene body methylation and the regulation of the zygotic genome in a wasp.
Article in PLoS genetics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- Gene body methylation suppresses intragenic transcription and permits epigenetic inheritance in a cnidarian.Nature ecology & evolution · 2026Article
- Genome-wide demethylation and targeted remethylation during metamorphosis in the jewel wasp Nasonia vitripennis.Epigenetics & chromatin · 2025Article
- Dnmt1 is required for early embryo development in the haplodiploid insect, Bemisia tabaci (Hemiptera: Aleyrodidae).Journal of insect science (Online) · 2025Article
- Unexpectedly low recombination rates and presence of hotspots in termite genomes.Genome research · 2025Article
- The functions of DNA methyltransferases during the feeding and development of Haemaphysalis longicornis are potentially associated with lysosome pathways.BMC genomics · 2024Article
- Epigenetics Research in Evolutionary Biology: Perspectives on Timescales and Mechanisms.Molecular biology and evolution · 2024Review
- Annelid methylomes reveal ancestral developmental and aging-associated epigenetic erosion across Bilateria.Genome biology · 2024Article
- Exploring the ageing methylome in the model insect, Nasonia vitripennis.BMC genomics · 2024Article
- Potential Role of DNA Methylation as a Driver of Plastic Responses to the Environment Across Cells, Organisms, and Populations.Genome biology and evolution · 2024Article
- 5mC modification orchestrates choriogenesis and fertilization by preventing prolonged ftz-f1 expression.Nature communications · 2023Article
- Neuromodulators and neuroepigenetics of social behavior in ants.Annals of the New York Academy of Sciences · 2023Review
- The role of Dmnt1 during spermatogenesis of the insect Oncopeltus fasciatus.Epigenetics & chromatin · 2023Article
- DNMT1 mutant ants develop normally but have disrupted oogenesis.Nature communications · 2023Article
- Review
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Gene body methylation (GBM) is an ancestral mode of DNA methylation whose role in development has been obscured by the more prominent roles of promoter and CpG island methylation. The wasp Nasonia vitripennis has little promoter and CpG island methylation, yet retains strong GBM, making it an excellent model for elucidating the roles of GBM. Here we show that N. vitripennis DNA methyltransferase 1a (Nv-Dnmt1a) knockdown leads to failures in cellularization and gastrulation of the embryo. Both of these disrupted events are hallmarks of the maternal-zygotic transition (MZT) in insects. Analysis of the embryonic transcriptome and methylome revealed strong reduction of GBM and widespread disruption of gene expression during embryogenesis after Nv-Dnmt1a knockdown. Strikingly, there was a strong correlation between loss of GBM and reduced gene expression in thousands of methylated loci, consistent with the hypothesis that GBM directly facilitates high levels of transcription. We propose that lower expression levels of methylated genes due to reduced GBM is the crucial direct effect of Nv-Dnmt1 knockdown. Subsequently, the disruption of methylated genes leads to downstream dysregulation of the MZT, culminating in developmental failure at gastrulation.
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