ArticleThe Journal of cell biology2022
Signaling by the integrated stress response kinase PKR is fine-tuned by dynamic clustering.
Article in The Journal of cell biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 46 citations in OpenAlex.
- Structural insights into HRI kinase activity and inhibition.Biochemical Society transactions · 2026Review
- PKR condensation at viral replication complexes initiates its activation.Cell reports · 2026Article
- RNA-binding proteins and ribonucleoproteins as determinants of immunity.Nature reviews. Immunology · 2026Review
- CSDE1 promotes viral immune evasion through RNA-dependent and phosphorylation-modulated liquid-liquid phase separation.Nature communications · 2026Article
- Review
- ADAR1 regulates dsRNA formation in nuclear and mitochondrial transcripts through editing-dependent and -independent mechanisms.Cell reports · 2026Article
- Toward unraveling molecular grammars for dsRNA-binding proteins: substrate recognition to binding mechanisms.BMB reports · 2025Review
- Mitochondria-Nuclear Crosstalk: Orchestrating mtDNA Maintenance.Environmental and molecular mutagenesis · 2025Review
- Effective recognition of double-stranded RNA does not require activation of cellular inflammation.Science advances · 2025Article
- Cooperative role of PACT and ADAR1 in preventing aberrant PKR activation by self-derived double-stranded RNA.Nature communications · 2025Article
- The integrated stress response engages a cell-autonomous, ligand-independent, DR5-driven apoptosis switch.Cell death & disease · 2025Article
- Catch me if you can: viral nucleic acids to host sensors.Frontiers in immunology · 2025Review
- A closer look at mammalian antiviral condensates.Biochemical Society transactions · 2024Review
- Condensation of RNase L promotes its rapid activation in response to viral infection in mammalian cells.Science signaling · 2024Article
- Crosstalk between vault RNAs and innate immunity.Molecular biology reports · 2024Review
- G3BP1-dependent condensation of translationally inactive viral RNAs antagonizes infection.Science advances · 2024Article
- The competitive landscape of the dsRNA world.Molecular cell · 2024Review
- Understanding the impact ofFrontiers in molecular biosciences · 2024Review
- DELE1 oligomerization promotes integrated stress response activation.Nature structural & molecular biology · 2023Article
- Surviving and Adapting to Stress: Translational Control and the Integrated Stress Response.Antioxidants & redox signaling · 2023Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The double-stranded RNA sensor kinase PKR is one of four integrated stress response (ISR) sensor kinases that phosphorylate the α subunit of eukaryotic initiation factor 2 (eIF2α) in response to stress. The current model of PKR activation considers the formation of back-to-back PKR dimers as a prerequisite for signal propagation. Here we show that PKR signaling involves the assembly of dynamic PKR clusters. PKR clustering is driven by ligand binding to PKR's sensor domain and by front-to-front interfaces between PKR's kinase domains. PKR clusters are discrete, heterogeneous, autonomous coalescences that share some protein components with processing bodies. Strikingly, eIF2α is not recruited to PKR clusters, and PKR cluster disruption enhances eIF2α phosphorylation. Together, these results support a model in which PKR clustering may limit encounters between PKR and eIF2α to buffer downstream signaling and prevent the ISR from misfiring.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.