ArticleFrontiers in cellular and infection microbiology2022
Elimination of Carryover Contamination in Real-Time Reverse Transcriptase Loop-Mediated Isothermal Amplification for Rapid Detection of the SARS-CoV-2 Virus in Point-of-Care Testing.
Article in Frontiers in cellular and infection microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 13 citations in OpenAlex.
- Development and clinical validation of a colorimetric isothermal assay for rapid detection of monkeypox virus.New microbes and new infections · 2026Article
- Enhanced serodiagnosis of opisthorchiasis using a multi-epitope dot-ELISA: comparative evaluation of visual and imageJ-assisted analysis of IgG and IgM responses.BMC infectious diseases · 2026Article
- A Systematically Enhanced LAMP Chip for Rapid, Sensitive, and Contamination-Free Pathogen Detection.ACS measurement science au · 2026Article
- Next-Generation Nucleic Acid-Based Diagnostics for Viral Pathogens: Lessons Learned from the SARS-CoV-2 Pandemic.Microorganisms · 2025Review
- Loop-Mediated Isothermal Amplification for Diagnosis of Zoonotic Malaria.The American journal of tropical medicine and hygiene · 2024Article
- Schistosomiasis diagnosis: Challenges and opportunities for elimination.PLoS neglected tropical diseases · 2024Review
- Development of a rapid and highly sensitive nucleic acid-based diagnostic test for schistosomes, leveraging on identical multi-repeat sequences.Frontiers in parasitology · 2024Article
- Development of a loop-mediated isothermal amplification (LAMP)-based electrochemical test for rapid detection of SARS-CoV-2.iScience · 2023Article
- Carryover Contamination-Controlled Amplicon Sequencing Workflow for Accurate Qualitative and Quantitative Detection of Pathogens: a Case Study on SARS-CoV-2.Microbiology spectrum · 2023Article
- Development and Validation of Rapid Colorimetric Reverse Transcription Loop-Mediated Isothermal Amplification for Detection of Rift Valley Fever Virus.Advances in virology · 2023Article
- Point-of-care system for rapid real-time detection of SARS-CoV-2 virus based on commercially available Arduino platforms.Frontiers in bioengineering and biotechnology · 2022Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Loop-mediated isothermal amplification (LAMP) is being used as a robust rapid diagnostic tool to prevent the transmission of infectious diseases. However, carryover contamination of LAMP-amplified products originating from previous tests has been a problem in LAMP-based bio-analytical assays. In this study, we developed a Cod-uracil-DNA-glycosylase real-time reverse transcriptase LAMP assay (Cod-UNG-rRT-LAMP) for the elimination of carryover contamination and the rapid detection of SARS-CoV-2 in point-of-care (POC) testing. Using the Cod-UNG-rRT-LAMP assay, the SARS-CoV-2 virus could be detected as low as 2 copies/µl (8 copies/reaction) within 45 min of amplification and 2.63 ± 0.17 pg (equivalent to 2.296 × 10
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