Evidence map›Paper›PMID 35521186›Full record

ArticleRSC advances2019

A lysosome-targetable fluorescent probe for the simultaneous sensing of Cys/Hcy and GSH from different emission channels.

Hui Zhang, Lizhen Xu, Wenxiu Li, Wenqiang Chen, Qi Xiao, Jun Huang, Chusheng Huang, Jiarong Sheng, Xiangzhi Song

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in RSC advances, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.9field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 19 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Hui ZhangCollege of Chemistry and Materials Science, Guangxi Key Laboratry of Natural Polymer Chemistry and Physics, Nanning Normal University Nanning 530001 P. R. China chenwq@csu.edu.cn +86 771 3908065 +86 771 3908065.
Lizhen XuCollege of Chemistry and Materials Science, Guangxi Key Laboratry of Natural Polymer Chemistry and Physics, Nanning Normal University Nanning 530001 P. R. China chenwq@csu.edu.cn +86 771 3908065 +86 771 3908065.
Wenxiu LiState Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources of Education Ministry, Guangxi Normal University 541004 Guilin Guangxi P. R. China.
Wenqiang ChenCollege of Chemistry and Materials Science, Guangxi Key Laboratry of Natural Polymer Chemistry and Physics, Nanning Normal University Nanning 530001 P. R. China chenwq@csu.edu.cn +86 771 3908065 +86 771 3908065.ORCID https://orcid.org/0000-0002-3404-9852
Qi XiaoCollege of Chemistry and Materials Science, Guangxi Key Laboratry of Natural Polymer Chemistry and Physics, Nanning Normal University Nanning 530001 P. R. China chenwq@csu.edu.cn +86 771 3908065 +86 771 3908065.ORCID https://orcid.org/0000-0002-6547-7411
Jun HuangCollege of Chemistry and Materials Science, Guangxi Key Laboratry of Natural Polymer Chemistry and Physics, Nanning Normal University Nanning 530001 P. R. China chenwq@csu.edu.cn +86 771 3908065 +86 771 3908065.
Chusheng HuangCollege of Chemistry and Materials Science, Guangxi Key Laboratry of Natural Polymer Chemistry and Physics, Nanning Normal University Nanning 530001 P. R. China chenwq@csu.edu.cn +86 771 3908065 +86 771 3908065.
Jiarong ShengCollege of Chemistry and Materials Science, Guangxi Key Laboratry of Natural Polymer Chemistry and Physics, Nanning Normal University Nanning 530001 P. R. China chenwq@csu.edu.cn +86 771 3908065 +86 771 3908065.
Xiangzhi SongCollege of Chemistry & Chemical Engineering, Central South University Changsha Hunan 410083 P. R. China.ORCID https://orcid.org/0000-0002-4034-6386
Nanning Normal University · CNCentral South University · CNGuangxi Normal University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A lysosome-specific fluorescent probe, Lyso-AC, for biothiols was developed by incorporation of a 4-nitrophenol moiety into a coumarin dye. The Cys/Hcy-triggered substitution-rearrangement cascade, and GSH-induced substitution reaction lead to the corresponding blue emissive amino-coumarin and yellow emissive thiol-coumarin, thereby enabling Cys/Hcy and GSH detection from distinct emissions. Moreover, this probe displayed an excellent lysosome targeting property with a 0.92 Pearson's colocalization coefficient by using Neutral Red as a reference. Significantly, biological experiments indicated Lyso-AC has the potential to monitor lysosome Cys/Hcy and GSH simultaneously in living HeLa cells from distinct emissions.

Identifiers

PMID35521186
PMCPMC9061761
OpenAlexW2922207369

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.