Evidence map›Paper›PMID 35520868›Full record

ArticleRSC advances2020

Cell-free protein synthesis and

Tony Köhler, Thomas Heida, Sandra Hoefgen, Niclas Weigel, Vito Valiante, Julian Thiele

Open access · goldAbstract read
In one paragraph

Article in RSC advances, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.8field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. Compartmentalized Cell-Free Expression Systems for Building Synthetic Cells.Advances in biochemical engineering/biotechnology · 2023
    Article
  3. Hydrogel-Based Multi-enzymatic System for Biosynthesis.Advances in biochemical engineering/biotechnology · 2023
    Review
  4. Solid-Phase Cell-Free Protein Synthesis and Its Applications in Biotechnology.Advances in biochemical engineering/biotechnology · 2023
    Article
  5. Review
  6. Review
  7. Effective Use of Linear DNA in Cell-Free Expression Systems.Frontiers in bioengineering and biotechnology · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Tony KöhlerInstitute of Physical Chemistry and Polymer Physics, Leibniz-Institut für Polymerforschung Dresden e.V. Hohe Str. 6 01069 Dresden Germany thiele@ipfdd.de.
Thomas HeidaInstitute of Physical Chemistry and Polymer Physics, Leibniz-Institut für Polymerforschung Dresden e.V. Hohe Str. 6 01069 Dresden Germany thiele@ipfdd.de.
Sandra HoefgenBiobricks of Microbial Natural Product Syntheses, Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology Adolf-Reichwein-Straße 23 07745 Jena Germany.
Niclas WeigelInstitute of Physical Chemistry and Polymer Physics, Leibniz-Institut für Polymerforschung Dresden e.V. Hohe Str. 6 01069 Dresden Germany thiele@ipfdd.de.
Vito ValianteBiobricks of Microbial Natural Product Syntheses, Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology Adolf-Reichwein-Straße 23 07745 Jena Germany.
Julian ThieleInstitute of Physical Chemistry and Polymer Physics, Leibniz-Institut für Polymerforschung Dresden e.V. Hohe Str. 6 01069 Dresden Germany thiele@ipfdd.de.ORCID https://orcid.org/0000-0001-5449-3048
Leibniz Institute of Polymer Research · DELeibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. - Hans-Knöll-Institut (HKI) · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the present work, microgels were utilized as a cell-free reaction environment to produce a functional malonyl-CoA synthetase (deGFP-MatB) under geometry-controlled transcription and translation. Our approach combines the straight-forward optimization of overall protein yield of an

Identifiers

PMID35520868
PMCPMC9057574
OpenAlexW3113405912

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.