Evidence map›Paper›PMID 35520043›Full record

ArticleRSC advances2020

Lignin nanoparticles are renewable and functional platforms for the concanavalin a oriented immobilization of glucose oxidase-peroxidase in cascade bio-sensing.

Eliana Capecchi, Davide Piccinino, Elisabetta Tomaino, Bruno Mattia Bizzarri, Francesca Polli, Riccarda Antiochia, Franco Mazzei, Raffaele Saladino

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in RSC advances, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
2.4field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 45 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 3 countries.

Eliana CapecchiDepartment of Biological and Ecological Sciences (DEB), University of Tuscia via S. Camillo de Lellis 01100 Viterbo Italy saladino@unitus.it.
Davide PiccininoDepartment of Biological and Ecological Sciences (DEB), University of Tuscia via S. Camillo de Lellis 01100 Viterbo Italy saladino@unitus.it.
Elisabetta TomainoDepartment of Biological and Ecological Sciences (DEB), University of Tuscia via S. Camillo de Lellis 01100 Viterbo Italy saladino@unitus.it.
Bruno Mattia BizzarriDepartment of Biological and Ecological Sciences (DEB), University of Tuscia via S. Camillo de Lellis 01100 Viterbo Italy saladino@unitus.it.ORCID https://orcid.org/0000-0001-7085-5432
Francesca PolliDepartment of Chemistry and Drug Technologies, Sapienza University of Rome P.le Aldo Moro 5 Rome 00185 Italy.
Riccarda AntiochiaDepartment of Chemistry and Drug Technologies, Sapienza University of Rome P.le Aldo Moro 5 Rome 00185 Italy.
Franco MazzeiDepartment of Chemistry and Drug Technologies, Sapienza University of Rome P.le Aldo Moro 5 Rome 00185 Italy.
Raffaele SaladinoDepartment of Biological and Ecological Sciences (DEB), University of Tuscia via S. Camillo de Lellis 01100 Viterbo Italy saladino@unitus.it.ORCID https://orcid.org/0000-0002-4420-9063
Università degli Studi della Tuscia · ITSapienza University of Rome · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Lignin nanoparticles (LNPs) acted as a renewable and efficient platform for the immobilization of horseradish peroxidase (HRP) and glucose oxidase (GOX) by a layer by layer procedure. The use of concanavalin A as a molecular spacer ensured the correct orientation and distance between the two enzymes as confirmed by Förster resonance energy transfer measurement. Layers with different chemo-physical properties tuned in a different way the activity and kinetic parameters of the enzymatic cascade, with cationic lignin performing as the best polyelectrolyte in the retention of the optimal Con A aggregation state. Electrochemical properties, temperature and pH stability, and reusability of the novel systems have been studied, as well as their capacity to perform as colorimetric biosensors in the detection of glucose using ABTS and dopamine as chromogenic substrates. A boosting effect of LNPs was observed during cyclovoltammetry analysis. The limit of detection (LOD) was found to be better than, or comparable to, that previously reported for other HRP-GOX immobilized systems, the best results being again obtained in the presence of a cationic lignin polyelectrolyte. Thus renewable lignin platforms worked as smart and functional devices for the preparation of green biosensors in the detection of glucose.

Identifiers

PMID35520043
PMCPMC9055843
OpenAlexW3047090794

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.