Evidence map›Paper›PMID 35519247›Full record

ArticleBiomedical optics express2022

Miniature structured illumination microscope for

Omkar D Supekar, Andrew Sias, Sean R Hansen, Gabriel Martinez, Graham C Peet, Xiaoyu Peng, Victor M Bright, Ethan G Hughes, Diego Restrepo, Douglas P Shepherd and 3 more

Open access · goldAbstract read
In one paragraph

Article in Biomedical optics express, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
11.9field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 45 citations in OpenAlex.

  1. Article
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  4. Miniaturized widefield microscope for high speed in vivo voltage imaging.bioRxiv : the preprint server for biology · 2025
    Article
  5. Voltage Imaging with Periodic Structured Illumination.bioRxiv : the preprint server for biology · 2025
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 1 country.

Omkar D SupekarDepartment of Electrical, Energy and Computer Engineering, University of Colorado Boulder, CO 80309, USA.ORCID https://orcid.org/0000-0003-1777-8508
Andrew SiasDepartment of Bioengineering, University of Colorado Anschutz Medical Campus, CO 80045, USA.
Sean R HansenDepartment of Bioengineering, University of Colorado Anschutz Medical Campus, CO 80045, USA.
Gabriel MartinezDepartment of Bioengineering, University of Colorado Anschutz Medical Campus, CO 80045, USA.
Graham C PeetDepartment of Cell and Developmental Biology, University of Colorado Anschutz Medical Campus, CO 80045, USA.
Xiaoyu PengDepartment of Physiology and Biophysics, University of Colorado Anschutz Medical Campus, CO 80045, USA.
Victor M BrightDepartment of Mechanical Engineering, University of Colorado Boulder, CO 80309, USA.
Ethan G HughesDepartment of Cell and Developmental Biology, University of Colorado Anschutz Medical Campus, CO 80045, USA.
Diego RestrepoDepartment of Cell and Developmental Biology, University of Colorado Anschutz Medical Campus, CO 80045, USA.
Douglas P ShepherdDepartment of Physics, Arizona State University, Tempe, AZ 85287, USA.ORCID https://orcid.org/0000-0001-9087-0832
Cristin G WelleDepartment of Physiology and Biophysics, University of Colorado Anschutz Medical Campus, CO 80045, USA.
Juliet T GopinathDepartment of Electrical, Energy and Computer Engineering, University of Colorado Boulder, CO 80309, USA.ORCID https://orcid.org/0000-0003-2387-5371
Emily A GibsonDepartment of Bioengineering, University of Colorado Anschutz Medical Campus, CO 80045, USA.
University of Colorado Anschutz Medical Campus · USUniversity of Colorado Boulder · USArizona State University · US

Funding

Shedding light on brain circuits mediating navigation of the odor plume in a natural environmentUF1NS116241 · NINDS · UNIVERSITY OF COLORADO DENVER · PI GIBSON, EMILY, RESTREPO, DIEGO · 2020 to 2021
$3.4M
Development of 3D-FAST Optical Interface for Rapid Volumetric Neural Sensing and ModulationR01NS123665 · NINDS · UNIVERSITY OF COLORADO DENVER · PI GIBSON, EMILY, WELLE, CRISTIN G · 2021 to 2021
$2.7M
3D-Fast Optical Interface for Rapid Volumetric Neural Sensing and ModulationR21EY029458 · NEI · UNIVERSITY OF COLORADO DENVER · PI GIBSON, EMILY, WELLE, CRISTIN G · 2018 to 2019
$479k
NEI NIH HHS R21 EY029458NINDS NIH HHS R01 NS123665NINDS NIH HHS UF1 NS116241
6 · The paper itself

Abstract

We present a high-resolution miniature, light-weight fluorescence microscope with electrowetting lens and onboard CMOS for high resolution volumetric imaging and structured illumination for rejection of out-of-focus and scattered light. The miniature microscope (SIMscope3D) delivers structured light using a coherent fiber bundle to obtain optical sectioning with an axial resolution of 18 µm. Volumetric imaging of eGFP labeled cells in fixed mouse brain tissue at depths up to 260 µm is demonstrated. The functionality of SIMscope3D to provide background free 3D imaging is shown by recording time series of microglia dynamics in awake mice at depths up to 120 µm in the brain.

Identifiers

PMID35519247
PMCPMC9045919
OpenAlexW4220882506

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.