Evidence map›Paper›PMID 35519051›Full record

ArticleRSC advances2020

Automated multiplex nucleic acid tests for rapid detection of SARS-CoV-2, influenza A and B infection with direct reverse-transcription quantitative PCR (dirRT-qPCR) assay in a centrifugal microfluidic platform.

Minghui Ji, Yun Xia, Jacky Fong-Chuen Loo, Lang Li, Ho-Pui Ho, Jianan He, Dayong Gu

Open access · goldAbstract read
In one paragraph

Article in RSC advances, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
0.9field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 57 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 5 institutions in 2 countries.

Minghui JiSchool of Nursing, Nanjing Medical University Nanjing 211166 P. R. China.
Yun XiaShenzhen International Travel Health Care Center, Shenzhen Academy of Inspection and Quarantine Shenzhen Customs District Shenzhen 518033 P. R. China hejianan6398@163.com.
Jacky Fong-Chuen LooDepartment of Biomedical Engineering, The Chinese University of Hong Kong Hong Kong SAR P. R. China jackyfcloo@link.cuhk.edu.hk.ORCID https://orcid.org/0000-0002-0355-8865
Lang LiShenzhen Bao'an Traditional Chinese Medicine Hospital (Group), Guangzhou University of Chinese Medicine Shenzhen 518133 P. R. China.
Ho-Pui HoDepartment of Biomedical Engineering, The Chinese University of Hong Kong Hong Kong SAR P. R. China jackyfcloo@link.cuhk.edu.hk.ORCID https://orcid.org/0000-0001-5001-5911
Jianan HeShenzhen International Travel Health Care Center, Shenzhen Academy of Inspection and Quarantine Shenzhen Customs District Shenzhen 518033 P. R. China hejianan6398@163.com.
Dayong GuDepartment of Laboratory Medicine, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Health Science Center Shenzhen 518035 P. R. China wanhood@163.com.
Chinese University of Hong Kong · HKShenzhen International Travel Health Care Center · CNGuangzhou University of Chinese Medicine · CNNanjing Medical University · CNShenzhen University Health Science Center · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The coronavirus disease 2019 (COVID-19) pandemic, caused by the new severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus, has posed a threat to public health worldwide. Also, influenza virus has caused a large number of deaths annually. Since co-infection of SARS-CoV-2 and influenza virus, which share similar symptoms, hampers current treatment efficiency, multiple simultaneous detection of these viruses is needed to provide the right treatment for patients. We developed a microfluidic disc-direct RT-qPCR (dirRT-qPCR) assay for rapid multiplex detection of SARS-CoV-2, influenza A and B viral infection in pharyngeal swab samples in an automated manner. Choices of the DNA polymerase, concentrations of dTPs and MgCl

Identifiers

PMID35519051
PMCPMC9056731
OpenAlexW3086349258

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.